Executor plant resistance proteins - pathway dissection by forward and reverse genetics

Executor R蛋白是一类新型的、结构多样化的蛋白质，可以触发植物免疫反应，但在机制上与众所周知的NLR型R蛋白不同。我们的目标是确定执行R蛋白的途径组件，以获得第一次深入了解其功能模式。我们将重点分析Bs 3和Bs 4C，辣椒的两个执行器R蛋白。辣椒突变体植物的人口将作为一个共同的平台，互补的正向和反向遗传筛选，旨在确定所需的Bs 3/Bs 4C介导的免疫反应的组件。前向筛选应通过辣椒突变群体的表型筛选来鉴定途径组分。随后，将通过测序定位鉴定因果突变。在反向筛选中，转录组分析将鉴定推定的途径组分。这些将在TILLING鉴定的相应辣椒突变体植物中进行功能研究。一旦我们确定了Bs 3/Bs 4C通路的组成部分，我们将阐明它们如何参与给定的免疫反应。我们的项目将提供第一个深入了解执行R蛋白Bs 3和Bs 4C如何介导免疫反应，以及不同的执行R蛋白和NLR型R蛋白是否具有共同的信号传导成分。

Executor R proteins are a novel, structurally diverse class of proteins that trigger plant immune reactions but that mechanistically differ from well-known NLR-type R proteins. We aim to identify pathway components of executor R proteins to get first insights into their mode of function. We will focus on the analysis of Bs3 and Bs4C, two executor R proteins from pepper. A population of pepper mutant plants will serve as a common platform for complementary forward and reverse genetic screens aimed at identifying components required for Bs3/Bs4C-mediated immune reactions. A forward screen shall identify pathway components by phenotypical screening of the pepper mutant population. Subsequently, causal mutations will be identified by mapping-by-sequencing. In the reverse screen, transcriptome-profiling will identify putative pathway components. These will be functionally studied in corresponding pepper mutant plants identified by TILLING. Once we have identified Bs3/Bs4C pathway components we will clarify how they are involved in the given immune reactions. Our project will provide the first insights into how the executor R proteins Bs3 and Bs4C mediate immune reactions and whether or not distinct executor R proteins and NLR-type R proteins share common signaling components.