Rodent models in psychiatry: severity assessment and potential measures for refinement and reduction

该项目旨在将严重性评估获得的所有结果整合到来自所有项目组的不同数据科学分析中，以确定来自FOR 2951联盟的所有模型的最稳健参数和直接可比性，并寻找改进策略。我们在精神病学研究中对小鼠和大鼠的严重程度进行了评估。我们的测量包括用于确定压力的常用参数，如行为异常和生理影响，如压力激素释放、体重和心率。使用新开发的数据科学方法，我们的目标是最稳健和有意义地代表动物压力的参数。这项调查是在精神病学模型中进行的，但也与研究小组的其他成员进行了总体比较。通过这种方式，可以澄清，例如，哪些测量变量特别合适，以及其他参数是否可能与精神病学动物研究相关，而不是其他模型。此外，我们正计划对一些特别有前途的测量参数进行meta分析，如体重、挖洞或筑巢行为，以便具体检查它们的稳健性，并最终检查它们的普遍性。我们将促进在FOR 2591框架内对先前结果的比较，并将能够使用新的统计方法直接比较结果，例如相对严重程度评估分数。这将使我们能够比较精神病学研究和其他神经科学研究的方法，而且，基于动物特定参数。这既适用于整个模型，也适用于单个动物。在多实验室的方法中，我们一方面在新的for 2591家庭系统中进行了遥测植入应力，另一方面，与项目的其他部分并行，研究了精神病学研究中的经典方法，如强迫游泳测试和恐惧条件反射。由于在即将进行的分析中几乎相同的测量，我们期望模型之间具有非常高的可比性。此外，我们正在研究使用homeage系统的可能性，以避免未来的侵入性植入，并在很长一段时间内使用自动分析行为。作为植入项目的一部分，我们也在研究镇痛药的改进。此外，我们还参与了系统和范围的文献综述。我们将使用强迫游泳测试的数据库，对测试本身的使用、测试方案和改进测试的可能方法进行说明。我们还将参与有关水和食物限制的文献分析。

This project aims to integrate all the results obtained on severity assessments into different data-scientific analyses from all project groups, in order to determine the most robust parameters and the direct comparability of all models from the FOR 2951 consortium, and to search for refinement strategies as well. We contribute the part on severity assessment of mice and rats in psychiatric research. Our measurements include the common parameters used to determine stress, such as behavioural abnormalities and physiological effects like stress hormone release, body weight and heart rates. Using a newly developed data science approach, we aim to target the parameters that most robustly and meaningfully represent stress in animals. This investigation takes place within the psychiatric models, but also in an overall comparison with the rest of the research group. In this way, it can be clarified, for example, which measurement variables are particularly suitable and whether other parameters may be relevant for psychiatric animal research than for other models. In addition, we are planning several meta-analyses on the particularly promising measurement parameters, such as body weight, burrowing or nest-building behaviour, in order to specifically check their robustness and ultimately also their generalisability. We will facilitate the comparison of previous results within the framework of FOR 2591 and will be able to directly compare the results using new statistical methods, for example the Relative Severity Assessment Score. This will allow us to compare methods from psychiatric research with those from other neuroscientific research and, moreover, on the basis of animal-specific parameters. This is done both for whole models, but also at the individual animal level. In a multi-laboratory approach, we have, on the one hand, carried out implantation stress for telemetry in the new FOR 2591 homecage system, and on the other hand, in parallel to the other parts of the project, investigated classical methods in psychiatric research, such as the forced swim test and fear conditioning. We expect a very high comparability between the models due to the almost identical measurement in the pending analyses. In addition, we are investigating the possibility of using the homecage system to avoid invasive implantation in the future and to use automated analyses of behaviour over long periods of time. As part of the implantation project, we are also contributing to studies on the refinement of analgesics. In addition, we participate in systematic and scoping literature reviews. We will use our database on the Forced Swim Test to make statements about the use of the test itself, the protocols and possible approaches to refinement of the test. We will also participate in the analysis of literature on water and food restriction.