Prokaryotic to plant phytochrome function (B08*)

我们建议将我们的光敏色素结构/功能的研究从蓝藻光敏色素Cph 1植物光敏色素，因为这些连接到完全不同的细胞信号通路。质子化动力学和Pr ParticipPfr光转换过程中的结构变化的作用将通过X射线和中子晶体学，固态核磁共振和表面标记方法来监测质子释放和结构域的变化，从而突变体将提供访问实验操作的分子信号机制。

We propose to extend our phytochrome structure/function studies from the cyanobacterial phytochrome Cph1 to plant phytochromes, as these connect to entirely different cellular signaling pathways. The role of protonation dynamics and structural changes during Pr↔Pfr photoconversion will be studied by X-ray and neutron crystallography, solid-state NMR and surface labeling methods to monitor proton release and domain shifts, whereby mutants will provide access to experimental manipulation of the molecular signaling mechanism.