Cell Differentiation by DNA Quaternary Complex to Nucleus Event

从烷基与DNA沟相互作用修饰DNA的方法出发，建立了DNA“单离子复合物”的新概念。 该复合物对小鼠胫骨肌细胞的转染活性明显高于临床裸DNA和市售的体内转染试剂jet PEI，对有丝分裂少的小鼠胫骨肌细胞的转染结果表明外源基因转染有可能保留其核膜。 因此，所得到的控制核事件的工具已经被建立，用于实现无有丝分裂的细胞的分化。

The new concept of DNA "mono-ion complex" had been established from the DNA modification method by the interaction of the alkyl group with the DNA groove. The resulting mono-ion complex has exhibited significant higher transfection activity for tibialis muscle than clinical naked DNA and commercial in vivo transfection reagent jet PEI.The achievement of transfection to mouse tibialis muscle cells with less mitosis suggests the possibility of exogenous gene transfection preserving their nucleus membrane. Therefore, the resulting tool to control nucleus event has been established for realizing the differentiation of cell with no mitosis.