Construction of protein network on DNA nanostrucuture as the novel nanoreactor

结构DNA纳米技术，其中包括DNA折纸，使自组装纳米结构的快速生产。该技术的关键特征之一是，各种形状和几何形状的完全可寻址的纳米架构很容易设计和构建。通过利用它们的可寻址性质，DNA纳米结构已被用作功能实体（如小分子和纳米颗粒）的定点组装的支架。除了这些功能实体之外，生物大分子（如蛋白质）由于其巨大的功能可变性而成为特别有趣的一类分子。在本研究中，我们在DNA折纸上安排了酶和蛋白质，并在DNA折纸上作为分子交换机或纳米反应器来证实它们的功能和合成。

Structural DNA nanotechnology, which includes DNA origami, enables the rapid production of self-assembled nanostructures. One of the key features of this technology is that fully addressable nanoarchitectures of various shapes and geometries are easily designed and constructed. By taking advantage of their addressable nature, DNA nanostructures have been used as scaffolds for the site-directed assembly of functional entities, such as small molecules and nanoparticles. As well as these functional entities, bio-macromolecules such as proteins are a particularly interesting class of molecules to assemble because of their huge functional variability. In this research, we arranged enzymes and on DNA-origami and the function and corabolation of them were confirmed on DNA-origami as the molecular switchboard or nano reactor.