Architecture and Function of the Epstein-Barr virus Replication Compartment

EB病毒（EBV）的生产性复制发生在细胞核中的离散位点，称为复制区室，其中发生病毒基因组DNA合成，转录和转录。复制区室包括亚核结构域，称为BMRF 1核心，其高度富集BMRF 1蛋白。在病毒裂解复制过程中，新合成的病毒DNA基因组围绕BMRF 1核心组织，然后储存在BMRF 1核心内。我们确定了复制区室中病毒早期和晚期基因mRNA的空间分布。EBV早期mRNA主要位于BMRF 1核心的外部，而病毒晚期mRNA则位于内部，这与晚期基因转录依赖于病毒DNA复制的事实相一致。此外，在BMRF 1-核心的最内部区域中发生了氢化。

Productive replication of the Epstein-Barr virus (EBV) occurs in discrete sites in nuclei, called replication compartments, where viral genome DNA synthesis, transcription, and encapsidation take place. The replication compartments include subnuclear domains, designated BMRF1 cores, which are highly enriched in the BMRF1 protein. During viral lytic replication, newly synthesized viral DNA genomes are organized around and then stored inside BMRF1 cores. We determined spatial distribution of viral early and late gene mRNAs within replication compartments. EBV early mRNAs were mainly located outside the BMRF1 cores, while viral late mRNAs were identified inside, corresponding well with the fact that late gene transcription is dependent on viral DNA replication. Further, the encapsidation occurs inside in innermost areas of BMRF1-cores.