Visualizing membrane contact site between the ER and Golgi to analyze the functional kinetics

作为第一步，可视化推测的细胞内细胞器，如内质网和高尔基体之间的膜接触位点（MCS），我证明了这些细胞器膜紧密并列的位置的存在，通过邻近连接试验。接下来，我试图建立一个分裂DHFR系统，用于实时监测体内MCS。目前，已获得了相当好的结果，预计在不久的将来，通过提高灵敏度和阐明胆固醇转运的调节机制，可以实现在体内可视化MCS的原始目标。

As a first step to visualize speculated membrane contact sites (MCS) between intracellular organelles such as the endoplasmic reticulum and Golgi apparatus, I proved the presence of the sites where these organelles membranes are closely apposed by Proximity Ligation Assay. Next, I tried to establish a split-DHFR system for real-time monitoring of MCS in vivo. At present, fairly good results are obtained and it is expected in near future to achieve the original goal of visualizing MCS in vivo by increasing the sensitivities and to clarify the mechanisms by which the transport of cholesterol is regulated.