Development of radioimmunoassay of inhibin and clinical application

制备了针对纯化的牛脯氨酸和牛过氧化物抑制素的抗血清。我们现在已经建立了RIA系统，可用于测量循环猪、大鼠、奶牛、山羊和妇女外周血血清中的抑制素浓度。在周期猪、大鼠和山羊中，血清抑制素水平在卵泡期较高，然后在排卵前LH/FSH激增后急剧下降。这些动物在黄体期抑制素水平相对较低。在循环奶牛中，血清抑制素水平在排卵前LH/FSH激增后36 ~ 48小时达到最大值，此时血清黄体酮水平仍然较低。抑制素水平随后下降，并在黄体期保持相对较低水平。在女性的月经周期中，血清抑制素水平在黄体期相当高，这与迄今为止研究的其他哺乳动物不同。这表明黄体期抑制素的主要来源是黄体，至少在女性中是这样。应用人抑制素特异性RIA法测定190例妊娠5 ~ 40周正常孕妇血浆抑制素浓度。孕妇在整个孕期的平均血浆抑制素浓度远高于月经周期正常的非孕妇。为了测定抑制素的日释放量，对5名健康青年男性每小时采集血样，持续24小时。在傍晚和夜间，血清浓度相对较低。关于基础抑制素水平的年龄相关变化，在20多岁时观察到最高，随着年龄的增长而降低。研究了17头日本超排卵黑牛血浆抑制素浓度与胚胎产量的关系。发情期抑制素浓度与排卵次数呈显著相关。

Antisera have been raised against purified procine and bovine overian inhibin. We have now established RIA systems that can be use for the measurement of inhibin concentrations in peripheral sera of cyclic pigs, rats, cows, goats and women.1. In cyclic pigs, rats andgoats, serum inhibin levals are high during the follicular phase and then drop sharply after the preovulatory LH/FSH surge. In those animals inhibin levels are relatively low during luteial phase.2. In cycling cows, serum inhibin levelstreached a maximum 36 to 48 hours after the preovulatory LH/FSH surge, when serum progesterone levels remained low. Inhibin levels then dropped and remained relatively low during luteal phase.3. In menstrual cycles of women, serum inhibin levels were quite high during the luteal phase, in contrast to the other mammals examined thus far. This suggests that a majar source of inhibin during the luteal phase is the corpus luteum, at least in women.4. The plasma inhibin concentrations in 190 normal pregnant women at 5-40 weeks gestation were measured by a specific RIA for human inhibin. The average plasma inhibin concentrations in pregnant women throughout pregnancy were much higher than those in nonpregnant women with a normal menstrual cycle.5. To determine the diurnal release of inhibin, blood sample were collected every hour for 24 h, from five healthy young adults men. During the evenign and the night, serum concentations were relatively low. With regard to the age-related changes on basal inhibin levels, the the highest values were observed in the twenties, and lower values were found with aging.6. The relationships between plasma concentrations of inhibin and embryo production were examined in 17 superovulated Japanese Black cows. Significant correlation was observed between the concentration of inhibin at estrus and the number of ovulation.