Antigen-antibody system associated with non-A, non-B hepatitis.

本文试图建立非甲非乙型肝炎的抗原抗体系统。首先，我们从输血后非甲非乙型肝炎患者的肝脏中纯化了与非甲非乙型肝炎患者恢复期血清反应的抗原。将该抗原命名为AN 6520抗原，并建立了该抗原和抗体的EIA检测体系。非甲非乙型肝炎恢复期抗体阳性率明显增高。然而，进一步的研究表明，这种抗原-抗体系统与非甲、非乙型肝炎无直接相关性。另一种尝试是用EB病毒转化法从恢复期黑猩猩中克隆产生非甲非乙型肝炎相关抗体的B淋巴细胞。我们建立了针对微管聚集体的单克隆抗体，该抗体被认为对非A、非B感染的黑猩猩具有特异性。抗原的纯化和检测体系的建立正在进行中。

An attempt was made to establish the antigen-antibody system associated with non-A, non-B hepatitis. First we purified antigen reacted with convalescent serum of the non-A, non-B hepatitis patients from the liver of a patient posttransfusion non-A, non-B hepatitis. This antigen designated as AN6520 antigen and established assay system of this antigen and antibody by EIA. Hi-prevalance of antibody in the convalescent srea of non-A, non-B hepatitis was evident. However, further investigation disclosed this antigen-antibody system does not dierctly associated with non-A, non-B hepatitis. The other attempt to clone the B-lymphocyte producing the antibody associated with non-A, non-B hepatitis from convalescent chimpanzee was made by EB virus transformation method. We established monoclonal antibody directed microtubular aggregate which is thought as specific for non-A, non-B infected chimpanzee. Purification of the antigen and establishment of assay system are ongoing.