Preventive measurement for vaccine brak of Marek's Disease, Divelopment of the vaccine in particular

本研究的最终目的是研制马立克病（MD）疫苗，以防止MD引起的疫苗断裂。目前得到的结果如下：1)MD病毒（MDV）和火鸡疱疹病毒（HVT）的血清学分为3组。培养了许多能分泌单克隆抗体的杂交瘤作为接种物。通过使用这些抗体，可以鉴定从该领域分离的病毒的血清型。此外，我们还开发了几种针对MD淋巴瘤细胞的单克隆抗体，用于MD肿瘤细胞的鉴定。2)调查了日本鹌鹑的MD发病率和HVT疫苗接种效果。在许多鹌鹑中观察到MD特有的病理病变。羽毛毛囊中mmv特异性抗原的存在与淋巴瘤变化的发生率呈正相关，而HVT抗体的存在与淋巴瘤变化的发生率呈负相关。从鹌鹑中分离出的MDV为血清型1，对鸡具有高致病性。从田间鸡中也分离到了类似的病毒。3)从13只鸡和10只野鸡中分离出新型非致癌性MDV，以控制MD的断苗，是研制MD疫苗的必要条件。从鸡细胞中分离到13株病毒，经单克隆抗体鉴定均为血清2型。利用这些病毒，在不久的将来研制出更有效的抗MD疫苗断裂疫苗是可行的。

The final aim of the present study is to develop Marek's disease (MD) vaccine which prevents the vaccine break induced by MD. The following results were obtained at Present.1) The group of MD virus (MDV) and herpesvirus of turkey (HVT) is serologically devided into 3 groups. Many hybridoma secreting monoclonal antibody specific to each group or strain used as inoculum were developed. By using these antibodies, it became possible to identify the serotype of the viruses isolated from th field. Further, several monoclonal antibodies specific to MD lymphoma cells were developed for identification of MD tumor cells.2) The incidence of MD and effect of HVT vaccine in Japanese quails were investigated. Pathological lesions specific to MD were observed in many quails. There was a positive correlation between the incidence of lymphomatous changes and the prosence of MMV-specific antigen in feather follicle, and there was a negative correlation between the incidence of lympomatous changes and the presence of antibody against HVT on a flock basis. the MDV isolated from the quails belongs to serotype 1 and these were highly pathogenic to chickens. the similar virus was also isolated from chikens in field.3) It is essential to isolate the new type of non-oncogenic MDV for development of the MD vaccine to control the vaccine break of MD. Attempts were made to isolate the virus from 13 chickens and 10 pheasant. Thirteen viruses isolated from cell of th chickens, and all of the viruses belong to serotype 2 as identified by the monoclonal antibody.By using these viruses, it is feasible to develop the more effective vaccine fought against vaccine break of MD in the near future.