Development of Laser Photoacoustic Imaging Immunoassay Metchod and System

1)激光光声成像分析仪的研制在实验室外组装了分辨率为12微米的激光光声显微镜光学元件。它有两个可选的激光光源：He-Ne(633 Nm)和Ar离子(514.5-488 nm)。通过编程对单元格表格进行X-Y方向扫描，并通过A/D转换获得信号。由此获得的数据点为10000-30000个。利用S型校准曲线，将光声信号转换为稀有材料(人-型L链)的量。2)新免疫分析方法的研制(1)光声显影免疫分析采用免疫酶反应进行染色。用40微米分辨率分析了人胚胎组织(厚5微米的脾和胰腺)的横切面。用本研究研制的多项免疫反应试纸对人L链、L链和免疫球蛋白进行多项光声免疫分析。测定范围为100 nm/ml-100微克/毫升。该方法的试剂盒系统正在研究中。3)本研究偶然发现了新的研究领域。开展了X射线光声成像分析。该方法将开辟三维光声显微分析的新领域。

1)Instrumentation of Laser Photoacoustic Imaging AnalyzerThe optics of laser photoacoustic microscopy with resolution of 12 <micrn>m was assembled in out lab. which has two alternative laser sources of He-Ne(633nm) and Ar ion(514.5-488nm). Microcomputer was programmed to make scanning od the cell table in X-Y direction and to get the signal through A/D converter. The data points thus obtained are 10000-30000 in number. Using sigmoidal calibration curve, the photoacoustic signal was converted to the amount og the rarget material (human <lambda>-type L-chain). the setting of the accumulating area and the classigication of the amount by coloe were also possible by this new apparatus.2)Development of new immunoassay(1)photoacounstic imaging immunoassayImmuno-enzyme reaction was used for staining. The cross sections of human embryo tissues (spleen and pancreas(5 um thick) were analyzed with 40 <micrn>m resolution. The local and total amounts of the <lambda>-type L-chain were estimated.(2)Multi-item photoacoustic immunoassayHuman <lambda>-type L-chain,<kappa>-type L-chain, and igG were analyzed by the multi-item immuno-reaction test paper which was developed by this study. The determination range was 100nm/ml-100ug/ml. the kit system for this method are under the investigation.3)New Study Field Incidentally Occurred by This ResearchUsing this apparatus. X-ray photoacoustic imaging analysis was developed. This method will open the new field of 3-dimensional photoacoustic mictoanalysis.