automated peptide synthesizer

固相多肽的自动化合成是多肽研究小组实验工作的重要组成部分。在多肽合成过程中，合成了以氨基酸为组成单元的各种不同序列的多肽链。人工合成多肽的缺点是序列组装中可能出现错误，以及工作时间限制在正常工作日。相比之下，自动多肽合成仪按照程序连续工作，没有错误。此外，与人工合成相比，通过机器的并行化可以合成更多的多肽，由自动合成的多肽组装的多肽的结构不仅限于20个蛋白质来源的氨基酸，而且还可以使用其他构建块来合成。在现代多肽研究领域，需要更多和更多的多肽，自动化多肽合成仪在实验室中是最重要的。所需的具有指定功能的机器是研究的重要补充，并支持计划中的研究培训小组的博士生的教育和培训(生物活性多肽-合成和生物合成的创新方面)。该机器和已建立的技术将向区域技术工作组的所有成员开放。这一应用的背景是对作为生化反应中的多肽底物、用于结构生物学研究、生物物理特性以及作为扩展化学合成的起始材料的合成肽的高需求。

The automated solid phase peptide synthesis is an essential part of experimentally working peptide researcher groups. During the peptide synthesis various peptide chains of different sequence, consisting of amino acids as building blocks are synthetically assembled. The disadvantage of a manual synthesis of peptides are possible errors in the assembly of the sequence, and the restriction of working hours to a normal working day. In constrast, the automated peptide synthesizer does work continously and according to the program without mistakes. Furthermore, though the parallelization of the machine, higher numbers of peptides can be synthesized, as compared to the manual synthesis.The structures of the peptides assembled by an automated peptide synthesized are not only restricted to the 20 proteinogenic amino acids, but also other building blocks can be used for synthesis. In the area of modern peptide research, where higher numbers and amounts of peptides are required, an automated peptide synthesizer is of highest importance in the laboratory.The required machine with the indicated functions is an important complement for research and supports the education and training of doctoral students in a planned research training group (Bioactive Peptides - Innovative Aspects of Synthesis and Biosynthesis). The machine and the established technology will be made accessible to all members of the RTG. Background of this application is a high demand of synthetic peptides serving as peptide substrates on biochemical reactions, of for structural biology investigations, biophysical characterizations and as a starting material for an extended chemical synthesis.