Studies on the Pathogenesis of Trophoblastic Tumors by DNA Fingerprint and its Relationship With Growth Factor

我们利用小卫星DNA探针（DNA指纹）分析限制性片段长度多态性（RFLPs）来研究滋养细胞肿瘤的发病机制，以完成包膜分子、癌和嗜水性流产。全鼹鼠的多态性片段全部来自父系DNA，而一些父系DNA的多态性条带在臼齿DNA中没有观察到（胎盘1989,10,pp399）。另一方面，臼齿线粒体DNA遗传自母体卵子（Gynecol Oncol 1991,40, pp29）。我们的研究结果在分子水平上证实了完整的分子是由无核卵子与正常精子受精产生的。在嗜水性流产中，DNA指纹的多态性片段可以追溯到其亲本，这表明DNA指纹可以作为鉴别完整分子和嗜水性流产的有用工具（Am J Obstet gynecology, 1990, pp634）。更令人震惊的是，在某些绒毛膜癌病例中，RFLPs的遗传模式与完整分子中的遗传模式相同，而在其他病例中，肿瘤组织中的所有多态性片段与宿主DNA中的多态性片段相同（Cancer Res 1990,50, pp488）。这些结果表明，绒毛膜癌的发病机制各不相同，在某些情况下与完全葡萄胎完全不同。我们还研究了巨噬细胞集落刺激因子（M-CSF）及其受体c-fms原癌基因在人胎盘和蜕膜中的基因表达，以阐明该生长因子在滋养细胞增殖中的作用。通过Northern blot杂交在早期胎盘中检测到M-CSF和c-fms mrna，分别为4.7kb和3.9kb，随后在妊娠期间增加。这些结果表明，M-CSF深入参与滋养细胞的局部增殖和分化，该生长因子的自分泌或旁分泌系统可能在滋养细胞肿瘤的生长中起关键作用（Am J . repd Immunol 1990,24, pp99）。少

We have investigated the pathogenesis of trophoblastic tumors by analyzing restriction fragment length polymorphisms (RFLPs) using a minisatellite DNA probe (DNA fingerprint) to complete hydatidiform molecules, riocarcinoma, and hydrotropic abortus. The polymorphic fragments of the complete mole were all transmitted from the paternal DNA, while some of the polymorphic bands of paternal DNA were not observed in molar DNA (placenta 1989, 10, pp399). On the other hand, the molar mitochondrial DNA was inherited from maternal ovum (Gynecol Oncol 1991, 40, pp29). Our results verify that complete molecule results from the fertilization of an anuclear empty ovum with normal sperm at the molecular level. In the hydrotropic abortus, the polymorphic fragments of the DNA fingerprint could be traced back to its parent, indicating that the DNA fingerprint could be a useful tool for differential diagnosis between complete molecules and hydrotropic abortus (Am J Obstet Gynecol 1990, 163, pp634).Intere … More stingly, in some cases of choriocarcinoma, the pattern of inheritance of RFLPs was the same as that in the complete molecule, Whereas in others all the polymorphic fragments in tumor tissues were identical to those in the host DNA (Cancer Res 1990, 50, pp488). These results suggest that the pathogenesis of choriocarcinoma varies, being completely different from that of the complete hydatidiform mole in some cases.We also investigated the gene expression of the macrophage colony-stimulating factor (M-CSF) and its receptor, c-fms proto-oncogene, in human placenta and decidua in order to clarify the role of this growth factor in the proliferation of trophoblasts. M-CSF and c-fms mRNAs, 4.7kb and 3.9kb respectively, were detected by Northern blot hybridization in the early stage placenta and subsequently increased during pregnancy. These results indicate that M-CSF is deeply involved in the local proliferation and differentiation of trophoblasts and the auto- or paracrine system of this growth factor may play a key role of the growth of trophoblastic tumors (Am J Reprod Immunol 1990, 24, pp99). Less