The Effects of Inhalational Anesthetics on Cancer Cells-Carcinostatic Effects of Inhalational Anesthetics-

众所周知，吸入麻醉剂对免疫系统和其他几种类型的细胞有抑制作用。本研究观察了异氟醚、七氟烷、安氟醚和氟烷对体外和体内大鼠肝癌细胞的作用。暴露组和非暴露对照组之间没有显著差异，尽管暴露组的生存能力略有下降。在不同的挥发性麻醉剂作用下，存活率没有统计学意义上的差异。肝癌细胞被放置在大鼠皮下形成肿瘤。大鼠通过股静脉接受完全肠外营养，并接受气管切开以实现人工呼吸。一种挥发性麻醉药持续5天，对照组用BARBI…麻醉再来点金枪鱼。两组均接受肌松药治疗。测定两组给药前后肿瘤直径，比较变化率。各挥发性麻醉剂组与对照组比较差异无统计学意义。同样，在接受不同挥发性麻醉剂的大鼠之间，这一比例没有显著差异。在本研究中，无论是在体外还是在体内，挥发性麻醉剂都有抑制癌细胞的趋势，但没有统计学意义。未能取得良好结果的原因被认为是：在培养实验中，我们没有使用超过临床使用浓度的挥发性麻醉药；在活体大鼠实验中，麻醉药物浓度低，吸入时间短。与大鼠相比，人类对这些麻醉剂的耐受性要高得多，并且可以接受更广泛的治疗形式，如使用面罩重复麻醉。如果将它们与使用线性偏振近红外的热疗或利用内毒素诱导的肿瘤坏死因子的治疗结合使用，可以预期更好的疗效。我们计划研究这些联合疗法。较少

Inhalation anesthetics are known to have a suppressive effect on the immune system and several other types of cell. In this study, we investigated the action of volatile anesthetics isoflurane, sevoflurane, enflurane and halothane on rat hepatic cancer cells in vitro and in vivo.Hepatic cancer cell cultures were exposed to volatile anesthetics at three to five MAC for 24 hours in tissue culture boxes placed in a CO2 incubator. There was no significant difference between the exposed and non-exposed-control groups, although viability was slightly reduced in the exposed group. No statistically significant difference in viability was observed after exposure to different volatile anesthetics.Hepatic cancer cell were subcutaneously placed in rats to form tumors. Rats received total parenteral nutrition via the femoral vein and were subjected to tracheostomy to enable artificial respiration. One MAC of volatile anesthetics was **en for five days ; the control group was anesthetized with barbi … More turate. Both groups received muscular relaxant. The tumor diameter was determined before and after anesthetic administration in both groups to compare the rate of change. No significant differences were observed in rate between the control and each volatile anesthetic group. Similarly, the rates were not significantly different among rats receiving different volatile anesthetics.In this study, both in vitro and in vivo, volatile anesthetics tended to suppress cancer cells, but not to a statistically significant extent. The reasons for failing to obtain good results are considered to be : in the culture experiment we did not use concentration of volatile anesthetics exceeding those used in clinical situations ; in living rat experiments the anesthetic concentrations were low and inhalation time was short. Humans are able to tolerate much higher levels of these anesthetics than rat and can undergo a wider range of forms of treatment such as repeated anesthesia using a mask. Greater effectiveness can be expected by using them in combination with thermotherapy using linear polarized near infrared or therapies utilizing tumor necrosis factor induced by endotoxins. We plan to investigate these combined therapies. Less