Multicolor Cell sorter

在国际上和基尔的生物医学研究中，生物医学临床和基础研究日益集中于复杂生物系统和器官中单个细胞或细胞组的确切分子和功能特征。在基尔，主要关注的是炎症医学和精准医学。基尔在测序和各种组学技术领域也拥有杰出的专业知识。通过对要研究的细胞类型进行分类，这些技术在确定的(单个)细胞或组的分子分析中的应用将得到优化和加强。特别是在免疫系统和复杂的组织样本(患者)中，这是一个挑战和必要性，现代流式细胞仪方法可以理想地解决这一问题。这需要技术设备，允许使用最大数量的参数对细胞混合物中的单个细胞进行高分辨率分析，同时为后续的功能或分子分析步骤提纯定义的细胞群体或单个细胞。基尔目前没有足够的设备(一个已使用14年、技术有限的设备已不再由制造商维护，必须更换)。为了满足基尔基地的不同要求，必须购买一台具有4个激光、多达16个颜色参数和单个电池偏转的多色电池分选机。多维细胞分选和分析程序需要高昂的仪器和人员成本以及专门的和不断更新的技术诀窍。因此，该设备将被集成到免疫学研究所新创建的细胞学核心设施中。

Biomedical clinical and basic research is increasingly concentrating on the exact molecular and functional characterization of individual cells or cell groups within complex biological systems and organs, both internationally and as in biomedical research in Kiel. In Kiel, the main focus is on inflammatory medicine and precision medicine. Kiel also has outstanding expertise in the field of sequencing and various -omics technologies. The application of these technologies to the molecular analysis of defined (single) cells or groups is to be optimized and intensified by sorting the cell types to be investigated. Especially in the immune system and within complex tissue samples (patients) this is a challenge and necessity which can be ideally addressed by modern flow cytometric methods. This requires technical equipment that allows high-resolution analysis of single cell within cell mixtures using a maximum number of parameters and simultaneously purifying defined cell populations or individual cells for subsequent functional or molecular analysis steps. No adequate equipment is currently available in Kiel (a 14-year-old, technically limited device is no longer maintained by the manufacturer and must be replaced). In order to meet the variable requirements at the Kiel site, the purchase of a multi-colour cell sorter with 4 lasers, up to 16 colour parameters and single cell deflection is essential. Multidimensional cell sorting and analysis procedures require high instrumental and personnel costs as well as specialized and constantly updated know-how. Therefore, the device is to be integrated into a newly created cytometry core facility at the Institute of Immunology.