Morphological analysis of organizational principles of neuronal connections in the mammalian central nervous system

1.用辣根过氧化物酶逆行法观察到日本猴杏仁中央核向脊髓的直接投射。用逆行和顺行麦胚凝集辣根过氧化物酶的方法研究猫的辣根过氧化物酶，发现三叉神经脊束核背侧核、三叉神经脊束核和尾侧核中的一些神经元向耳蜗背、腹侧核发出投射纤维。在猫纹状体的投射神经元中可见神经降压素样免疫反应。其中一些具有神经降压素样免疫反应的神经元进一步被证明含有脑啡肽或伽马氨基丁酸。猫的丘脑味觉核(丘脑后内侧腹内侧核的腹内侧区)不仅向大脑皮层的味觉区(43区)发出投射纤维，而且还向杏仁外侧核背侧部、边缘下皮质(25区)和大脑皮质周围区(35区)发出投射纤维。细胞色素氧化酶组织化学显示猫三叉神经感觉主核腹侧亚核尾侧、三叉神经脊束间核和三叉神经脊束核尾侧IV层的酶活性呈斑片状。通过辣根过氧化物酶的跨神经节转运法，表明这些模式复制了面部触须的空间排列。

1. Direct projections from the central amygdaloid nucleus to the spinal cord were found in the Japanese monkey by the retrograde horseradish peroxidase method.2. A retrograde and anterograde wheat germ agglutinated horseradish peroxidase study in the cat revealed that some neurons in the dorsal column nuclei and interpolar and caudal spinal trigeminal nuclei sent projection fibers to the dorsal and ventral cochlear nuclei.3. Neurotensin-like immunoreactivity was visualized in projection neurons in the striatum of the cat. Some of these neurons with neurotensin-like immunoreactivity were further indicated to contain enkephalin or gamma amino butyric acid.4. The thalamic taste are (ventromedial region in the parvicellular division of the posteromedial ventral nucleus of the thalamus) in the cat were found to send projection fibers not only to the taste area in the cerebral cortex (area 43) but also to the rostrodorsal part of the lateral amygdaloid nucleus, infralimbic cortex (area 25), and perirhinal cortical area (area 35).5. Cytochrome oxidase histochemistry revealed patchy patterns of the enzyme activity in transverse sections through the caudal part of the ventral subnucleus of the main sensory trigeminal nucleus, interpolar spinal trigeminal nucleus, and layer IV of the caudal spinal trigeminal nucleus in the cat. By the transganglionic transport method of horseradish peroxidase, the patterns were indicated to replicate the spacial array of the facial vibrissae.