STUDIES ON THE DIFFERENTIATED FUNCTION AND GROWTH OF THYROID FOLLICULAR CELLS.

用人和动物甲状腺激素研究了甲状腺滤泡细胞的生长和分化功能。Fisher大鼠功能性甲状腺细胞(FRTL-5)在EGFBINDING结构域上存在异常，但用特异的CDNA探针和单抗证明它们具有EGF受体。从FRTL-5细胞中获得了8-氮杂鸟嘌呤抗性突变克隆。这些被应用于与几个人类细胞的细胞融合研究。研究了白细胞介素1α对小鼠甲状腺的影响。同时给予IL-1治疗7天。甲状腺功能严重受抑。再远一点。给予IL-1 3周后，甲状腺对TSH的获得性无反应性和垂体TSH含量增加。采用原代培养各种人甲状腺细胞，研究EGF受体的特性以及EGF和TSH的生长效应。癌细胞的结合亲和力明显较小，但对EGF的反应良好。正常(非肿瘤性)和良性肿瘤细胞EGF受体结合亲和力与外源性EGF掺入生长刺激因子3HTYMIDINE呈显著负相关。这些细胞已经产生了多种生长因子和细胞因子，如AD、转化生长因子α、IL-1α、脑脊液和甲状旁腺激素样物质。它们的生长在很大程度上依赖于血清成分，也受各种生长因子的影响。综上所述，关于甲状腺细胞的生长和分化功能，获得了许多新的证据。这些研究成果对今后甲状腺研究的发展和临床应用具有一定的参考价值。

CELL GROWTH AND DIFFERENTIATED FUNCTIONS OF THE THYROID FOLLICULAR CELLS WERE STUDIED USING HUMAN AND ANIMAL THYROIDS.FUNCTIONING FISHER RAT THYROID CELLS (FRTL-5) WERE FOUND TO HAVE ABNORMALITY ON THE EGFBINDING DOMAIN, BUT THEIR POSSESSION OF EGF-RECEPTOR WAS PROVED USING SPECIFIC CDNA PROBES AND MONOCLONAL ANTIBODIES. MUTANT CLONES WITH 8-AZAGUANINE RESISTANCE WERE OBTAINED FROM THE FRTL-5 CELLS. THESE WERE APPLIED TO THE CELL-FUSION STUDY WITH SEVERAL HUMAN CELLS. RESULTS OBTAINED WERE OF VERY INTRIGUING TO SEE THE DEVELOPMENT OF DIFFERENTIATED FUNCTIONS AND THEIR GENE EXPRESSION.EFFECTS OF INTERLEUKIN-1 ALPHA ON THE MOUSE THYROID WERE STUDIED. DURING ADMINISTRATION OF IL-1 FOR 7 DAYS. THE THYROID FUNCTIONS WERE GREATLY SUPPRESSED. FURTHER. AFTER 3 WEEKS OF IL-1 ADMINISTRATION, THE THYROID ACQUIRED UNRESPONSIVENESS TO TSH AND PITUITARY TSH CONTENTS WERE INCREASED.USING PRIMARY CULTURE OF VARIOUS HUMAN THYROCYTES, PROPERTIES OF EGF RECEPTOR AND GROWTH EFFECTS OF EGF AND TSH WERE STUDIED. CANCER CELLS HAD SIGNIFICANTLY SMALLER BINDING AFFINITY BUT WELL RESPONDED TO EGF. NORMAL (NON-NEOPLASTIC) AND BENIGN TUMOR CELLS WERE FOUND TO SHOW A CLOSE NEGATIVE CORRELATION BETWEEN BINDING AFFINITY OF EGF RECEPTOR AND GROWTH STIMULATORY 3HTHYMIDINE INCORPORATION BY EXOGENOUS EGF.A CELL LINE WAS ESTABLISHED FROM TUMOR CELLS OF A PATIENT WITH UNDIFFERENTIATED GIANT CELL THYROID CANCER. THESE CELLS HAVE BEEN PRODUCING SEVERAL GROWTH FACTORS AND CYTOKINES SUCH AD TGF-ALPHA, IL-1 ALPHA, CSF AND PTH LIKE SUBSTANCE. GROWTH OF THEM IS GREATLY DEPENDENT ON SERUM COMPONENT AND IS ALSO AFFECTED BY VARIOUS GROWTH FACTORS.IN SUMMARY, QUITE A FEW NOVEL EVIDENCES CONCERNING WITH THE THYROID CELL GROWTH AND THEDIFFERENTIATED FUNCTIONS WERE OBTAINED. MOST OF THEM WILL SURELY BE USEFUL FOR THE FUTURE DEVELOPMENT OF THE RESEARCHES AND THEIR CLINICAL APPLICATIONS IN THE THYROID STUDIES.