Clinical application of bioartificial mucosa.

本研究旨在构建生物人工黏膜修复口腔黏膜的新方法。本研究项目1988年至1989年的结果总结如下：1)用ix10”成纤维细胞和17%的胶原凝胶可构建人活细胞生物人工黏膜。组织学表现与正常人粘膜非常相似。2)用风干法检查培养细胞的染色体，确认其正常和安全。所有培养的细胞在几个月的培养期内均表现正常。3)采用透明电子显微镜观察基底膜，确定生物人工粘膜移植的最佳时机。结果表明，上皮细胞培养后第四周为移植的最佳时机。4)生物人工黏膜移植时，防止感染和干燥。为预防其发生，局部应用缓释抗生素。5)在此基础上，在名古屋大学医院进行了3例生物人工粘膜移植手术。在所有病例中，移植物都存活得很好。未观察到免疫排斥反应。

The purpose of this study was to construct the new method of oral mucosal repair with the bioartificial mucosa.The results of this research project through 1988 and 1989 were summarized as follows.1) The bioartificial mucosa using human living cells could be constructed with IX 10" fibroblasts and 17% collagen gel. The histological findings were very similar with normal human mucosa.2) The chromosome of cultured cells were checked by the air- dry- method to be confirmed their normality and safety. All cultured cells had normal findings within a few months culture periods.3) The most suitable timing of grafting of the bioartificial mucosa was decided to observed the basement membrane with transparent electron microscope. As the result the most suitable timing of grafting was the fourth week after the epithelial culturing.4) When the bioartificial mucosa was grafted, the protection from infection and drying. To prevent them the slow releasing antibiotics was applied locally.5) Based upon above results, 3 cases of bioartificial mucosal grafting were carried out at Nagoya University Hospital. In all cases the graft survived very well. No immunological rejection was observed.