Studies of the expression of cell adhesion-related molecules and cytoskeletal architecture in human meningioma cells

脑膜瘤通常是良性的。然而，即使在完全切除后，它们的复发率也有10%-30%，而且还有生物学侵袭性脑膜瘤。为了研究人脑膜瘤的细胞生物学，我们从转移性脑膜瘤来源的细胞(GMM-2)中分离到一株具有形成蛛网膜细胞分化标志--波形蛋白-细丝锚定桥粒的新的永生细胞系。我们从GMM-2细胞中获得了30多个克隆，但大多数克隆都衰老了。我们选择了一个命名为HMG-10的克隆进行进一步的研究，达到了100个种群倍增的水平。在对数生长期，HMG-10细胞形成上皮样细胞(E)片。13EA02：免疫细胞化学显示桥粒粘连蛋白阳性斑点排列在细胞与细胞交界处。细胞质网点被认为是内化的桥粒。胞核周围分布有Vimentin阳性细丝。肌动蛋白细丝主要分布在细胞周边。细胞角蛋白、上皮膜抗原和E-钙粘附素均为阳性。HMG-10细胞的电子显微镜下可见桥粒和大量胞浆IF。IF锚定桥粒不仅存在于E细胞之间，而且存在于E细胞与F细胞之间以及F细胞之间。在含10%胎牛血清的培养液中，细胞呈指数生长，第一次换液后倍增时间约为47h。在20000个/cm2的低密度下，HMG-10细胞的生长受到接触抑制。我们希望对HMG-10细胞黏附分子表达的进一步研究将为深入了解人脑膜瘤细胞的生长调控机制提供一些参考。

Meningiomas are generally benign. However, they recur with a rate of 10-30%, even after total resection, and there are biologically aggressiove meningiomas. To study the cell biology of human meningioma, we isolated a new immortal cell line with the ability to form vimentin-filament anchoring desmosome, a differentiation marker of arachnoid cell, from metastatic meningioma-derived cells (GMM-2). We obtained more than 30 clones from the GMM-2 cells, but most of the clones became senescent. We selected a clone designated HMG-10 for further study, which achived 100 population doubling level. In the log phase, HMG-10 cells formed epthelioid cell (E) sheets. After confuluence, part of them poorly spread, and appeared to bie fibroblastoid cells (F).13EA02 : Immunocytochemistry revealed that desmoplakin-positive dots were aligned at cell-to-cell boudaries. Cytoplasmic dots were considered intternalized desmosomes. Vimentin-positive filamants were distributed around the nucleus. Actin filaments were present mainly at the cell periphery. None of the cells were positive for cytokeratin, epithelial membrane antigen and E-cadherin. Electron microscopy of HMG-10 cells showed desmosomes and numerous cytoplasmic IF.IF anchoring desmosomes were assembled not only between E cells, but also between E cell and F cell as well as between F cells. In growth medium containing 10% fetal calf serum, the cells grew exponentially with a doubling time of about 47 hours after the first medium change. Their growth was contact-inhibited at a low density of 20,000 cells/cm^2. We are hopeful that further studies on the expression of cell adhesion molecules of HMG-10 cells will provide some insights into the growth control mechanism of human meningioma cells.