Detection of chromosome abnomality by fluorescent in situ hybridization in brain tumors.

（1）脑肿瘤细胞周期的流式细胞术分析流式细胞术（Flow cytometry，FCM）是研究脑肿瘤生物学特性的有效方法。我们可以阐明1-己氨甲酰-5-氟尿嘧啶、顺铂、MCNU和TNF等抗癌药物的作用机制。（2）脑肿瘤中癌基因产物的表达在已建立的脑肿瘤细胞株中，免疫组化和流式细胞术方法可用于评价c-myc产物的阳性表达。（3）染色体异常FISH检测方法的建立：1）用FISH方法检测胶质瘤细胞系中7、9、10、17号染色体数目; 2）手术切除的胶质瘤中7、17号染色体数目较高; 3）脑膜瘤染色体数目较低（22），复发病例染色体数目较高（22）。（4）石蜡包埋标本的染色体异常检测：成功地用石蜡包埋法检测出染色体数目，并与新鲜标本相对照。

(1)Flow cytometric analysis of the cell cycle in brain tumors.Flow cytometory (FCM) has been an effective method to abtain the biological aspects of brain tumors. We can clarify the mechanism of anticancer agents such as 1-hexycarbamoy 1-5 fluouracil, CDDP, MCNU and TNF.(2)Expression of oncogene products in brain tumors.In established brain tumor cells strains, immunohistochemical and flow cytometric methods are useful for evaluation of the positivity of c-myc products expression.(3)Development of FISH method for detection of chromosome abnomality.1)Chromosome numbers of 7,9,10,17 were dectected in glioma cell lines by FISH method.2)Chromosome numbers of 7,17 were high in gliomas obtained by surgical operation.3)Meningioma showed low chromosome numbers of 22, but high chromosome numbers of 22 in recurrent cases.(4)Detection of chromosome abnomality with use of paraffin embeded specimen.We succeeded to detect the chromosome numbers with use of paraffin blocks, which was correlated with fresh specimen.