DERIVATION OF HAIRY ROOTS EXPRESSING BIOLOGICAL REACTIONS IN LEAF TISSUE AND PRODUCTION OF USEFUL MATERIALS IN CULTURE WITH PHOTOBIOREACTOR

1.毛状根在连续光照下逐渐变绿，随着叶绿素的形成和细胞内类囊体膜系统的发育，毛状根保持了分枝根的形态。光照培养的绿色毛状根根系生长旺盛，超氧化物歧化酶（SOD）和过氧化物酶（POD）活性增强。在11.1 W/m^2光强条件下，绿毛根培养第21天的根系生长、总SOD活性和总POD活性分别为8.1g干根重/dm^3、18 × 10^4 U/dm^3和40 × 10^3 U/dm^3。暗培养第21天的相应值分别为3.8 g-干根重/dm^3、3.0 × 10^4 U/dm^3和9.5 × 10^3 U/dm^3。光照条件下，随着根系生长点数量的增加，促进了植株的生长。光照下毛状根叶绿素的形成，叶绿素含量随着离根尖距离的增加而增加，并逐渐接近饱和值。叶绿素含量的这种位置依赖性被认为是由于毛状根的线性生长导致的细胞年龄分布。在此基础上，建立了叶绿素形成与生长相关的动力学模型。细胞质量和叶绿素含量的计算值与实验值吻合较好，说明该模型可以很好地描述毛状根培养过程中生长和叶绿素形成的动力学行为。绿色的毛状根可以在内部照明的生物反应器中培养。由于反应器内光照强度的分布，细胞内叶绿素含量、SOD和POD活性随毛状根位置的变化而变化。

1.Hairy roots of pak-bung turned green when cultured under continuous light exposure, and maintained their branched root morphology with the formation of chlorophyll and the development of a thylakoid membrane system in the cells. Green hairy roots cultivated under light exhibited high root growth and enhanced activities of superoxide dismutase (SOD) and peroxidase (POD). In cultures of green hairy roots at a light intensity of 11.1 W/m^2, root growth, total SOD activity and total POD activity obtained on culture day 21 were 8.1g-dry root weight/dm^3,18x10^4 U/dm^3 and 40x10^3 U/dm^3, respectively. In dark culture, the corresponding values obtained on culture day 21 were 3.8 g-dry root weight/dm^3,3.0x10^4 U/dm^3 and 9.5x10^3 U/dm^3, respectively.2.Under the light condition, the growth was promoted accompanying the increase in number of growing points of roots. Concerning the chlorophyll formation in the hairy roots exposed to light, chlorophyll content increased with the increase in distance from the root tips and gradually approached a saturated value. This positional dependence of chlorophyll content was considered to arise from the distribution in cellular age due to a linear growth of the hairy roots. Based on these results, a kinetic model of chlorophyll formation associated with the growth was presented. The calculated values of cell mass and chlorophyll content were in fair agreement with experimental ones, indicating that the model made it possible to describe the kinetic behaviors of growth and chlorophyll formation in the hairy root cultures.3.Green hairy roots could be cultivated in an internally illuminated bioreactor. It was observed that the chlorophyll content, and SOD and POD activities in the cells changed depending on the location of the hairy roots because of the distribution of light intensity in the reactor.