Synthesis, transport and fate of G-protein coupled 7-transmembrane receptors

G蛋白偶联7跨膜受体的合成、转运和命运可以用神经元模型来分析。用抗GnRH受体和内皮素A型受体的抗体对成年雄性Wistar大鼠下丘脑和中脑进行免疫组化染色，并在光镜、电镜和激光共聚焦扫描显微镜下进行观察。通过配体给药分析这些受体的消耗或内化。在这些实验中，高尔基体出芽和轴突运输抑制brefeldin A和秋水仙碱，分别脑室注射。此外，我们观察了ET-A受体的免疫染色模式的时间过程中的变化，与儿茶酚胺合成酶，TH和DBH，在60 HDA给药的大鼠相比，我们的结论是，这种类型的受体是在rER-高尔基体系统中合成，纳入到膜，然后从高尔基体出芽囊泡膜运输到与细胞膜融合。

The synthesis, transport and fate of G-protein coupled 7-transmembrane receptors can be analyzed using neuron model. Because a neuron has perikaryon where receptor protein is synthesized, and long processes where receptors are functioning.The hypothalamus and mesencephalon of adult male Wistar rats were immunostained with the antibodies to GnRH receptor and endothelin type A receptor, and observed under light-, electron- and conforcal laser scan microscopes. The consumption or internalization of these receptors was analyzed by ligand administration. In these experiments, the Golgi-budding and axonal transport were inhibited by cerebroventricular injection of brefeldin A and colchicine, respectively. Moreover, we observed time-course change of immunostaining pattern of ET-A receptor in comparison with catecholamine synthesizing enzyme, TH and DBH,in the 60HDA administered rat.We conclude that this type receptor is synthesized in the rER-Golgi system, incorporated into the membrane, and then transported with vesicular membrane from Golgi-budding to fusion with the cell membrane.