400 MHz NMR console für solid-state-NMR

DNP增强的固态核磁共振使灵敏度提高了数量级。它为新的、非常规的核磁共振实验打开了大门，特别是对低自旋数样品的分析。我们特别将这项技术用于膜蛋白的研究，特别是用于探测大分子复合体中的亚基相互作用，分析低密度中间态以及具有转运体或受体的复合体中的小分子。感兴趣的蛋白质家族有GPCRs、ABC转运蛋白、激酶和光受体。为了继续和扩大这些研究，需要更换切除DNP-核磁共振光谱仪中过时的核磁共振控制台，这是本提案的目的。

DNP-enhanced solid-state NMR offers an increase in sensitivity by orders of magnitude. It opens the door to new, unconventional NMR experiments and especially the analysis of samples with low spin numbers. We use this technique especially for the study of membrane proteins and in particular for probing subunit interactions within large complexes, for analyzing low-populated intermediate states and for small molecules in complex with transporters or receptors. Protein families of interest are GPCRs, ABC transporter, kinases and photoreceptors. In order to continue and to expand these studies, an outdated NMR console within the excising DNP-NMR spectrometer needs to be replaced, which is the aim of this proposal.