Control of Nano-Reaction Field by Bio-Probe Nanolithography

构建分子装置是纳米技术的最大目标之一。纳米光刻技术对于这种分子器件的形成是必不可少的。扫描探针光刻(SPL)就是其中的一种纳米光刻技术。在本研究中，我们尝试用SPL将蛋白质等生物分子固定在纳米结构域中。首先，我们通过制备具有一定表面特征的自升单分子膜(SAM)制备了表面，并研究了蛋白质等生物分子在SAM上的吸附行为。结果表明，表面润湿性和表面电势、化学亲和力对生物分子的吸附行为有很大影响。其次，利用光刻技术制备了具有两个不同表面特征域(表面润湿、表面电势、化学亲和力)的微图案化表面，并观察了生物分子在其上的吸附行为。然后确认该吸附剂附着在微图案的特定结构域上。通过上述表面特性，我们成功地控制了吸附剂在特定表面的固定化。最后，我们利用SPL制备了具有不同表面特性的纳米颗粒表面。采用浸笔式纳米光刻技术(DPN)在硅衬底上构建了金纳米结构。然后，在结构体上吸附各种卷曲化合物，控制纳米结构域表面的表面特征。我们成功地实现了纳米粒子作为蛋白质和DNA的模型化合物的位置选择性固定化。

Construction of the molecular device is one of the biggest aims in nanotechnology. Nanolithography is essential to the formulation of such a molecular device. We have focused on Scanning Probe Lithography (SPL) as one of such nanolithographic techniques. In this study, we attempted to immobilize biomolecules such as proteins in nano-domains by SPL.At first, we have prepared surfaces with some surface characteristics by fabrication of self-ascembled monolayer (SAM) and investigated adsorption behaviors of biomolecules such as proteins on the SAMs. As a result, it was clarified that the surface wetting and surface potential, chemical affinity had great influences on the adsorption behavior of the biomolecules.Next, we have fabricated micropatterned surfaces with two different domains in the surface characteristics(surface wetting, surface potential, chemical affinity)by light lithography and observed adsorption behavior of biomolecules on them. Then it was confirmed that the adsorbent adhered to the specific domain of the micro-pattern. We successfully controlled the immobilization of the adsorbents on the specific surface by the surface characteristics mentioned above.Finally we have manufactured nanopatterned surfaces with different surface characteristics by SPL. Gold nanostructures on a silicon substrate were constructed by "Dip-Pen Nanolithography(DPN)"as one of SPL. Then, various kinds of frijol compounds were adsorbed on the structure body and surface characteristics of the nano-domain surface were controlled. We have successfully achieved position-selective immobilization of nanoparticles, as a model compound of a protein, and DNA.