Integrate research of genomics and proteomics of novel molecular targets for development of periodontal diseases cure

利用功能基因组学技术和DNA/蛋白质数据库进行的实验研究将有助于在转录水平上识别基因及其表达，举例说明它们对理解生命科学的作用。与口腔传染病相关的几个细菌基因组计划正在进行中。人类基因组已经被测序和组装，可以在不久的将来进行遗传学研究。近年来，消减基因克隆和DNA微阵列等技术的进步被应用于发现参与复杂代谢的新基因。例如，由于对牙龈上皮细胞在与牙周病原体相互作用后参与炎症反应的分子知之甚少，为了找到人牙龈上皮细胞对脂多糖的反应基因的转录图谱，我们使用超过8,000个基因芯片(Incell，GEM)检测了许多基因表达的变化。为了找出颌下腺基因随年龄增长的转录图谱，我们用核苷酸微阵列(Affimetrix，GeneChip)检测了超过6,500个基因的mRNA水平。我们的实验室参与了使用这些技术和基因组数据库的牙齿科学项目。在这笔赠款支持下，我们计划建立新的口腔疾病数据库，方便许多研究人员使用。基因组计划和利用数据库的分子生物学方法将打开发展牙科科学的大门。

Experimental research utilizing functional genomics technologies with DNA/protein database will serve to identification of genes and their expressions on the transcriptional level exemplify their utility for the understanding the life science. Several bacterial genome projects associated with oral infectious diseases are in progress. Human genome has been sequenced and assembled, making the accessible for genetic studies in near future. Recently, technological advances, such as subtractive gene cloning and DNA microarray, have been applied to discover of novel genes involved in complex metabolism. For example, since little is known regarding the molecules expressed by gingival epithelial cells that are involved in inflammation following the interaction with periodontal pathogens, to find transcriptional profiling of genes in human gingival epitherial cells in response to LPS, we examined many altered gene expressions using over 8,000 cDNA microarray (Incyte, GEM). To find transcriptional profiles of genes in submandibular gland by ageing, we examined mRNA levels of over 6,500 genes by nucleotide micreoarray (Affimetrix, GeneChip). Our laboratory involved in dental science projects using these technologies and genome database. By this Grant support, we plan to make the new database for oral diseases, which will be used easily by many researchers. The genome project and molecular biological approaches using the database will open the gate develop the dental science.