Analysis on the higher-order chromatin structure in the interphase nucleus using super-resolution fluorescence microscopy

我开发了一种使用超分辨率显微镜进行活体观察的方法，3DSIM，并将其应用于活的分裂酵母。裂解酵母中的间期染色质形成纤维状结构，直径100~150 nm。将固定的裂解酵母细胞核中的染色质用抗修饰组蛋白的抗体进行染色，并用3DSIM观察，然后定量分析与DNA质量的重叠程度。结果表明，与正在进行的转录相关的区域从纤维结构解聚和延伸。

I developed a method for live observation using super-resolution microscopy, 3DSIM, and applied on live fission yeast. The interphase chromatin in live fission yeast formed fibrous structures with a diameter of 100-150 nm. Chromatin in fixed fission yeast nuclei was stained with antibodies against modified histones and observed with 3DSIM, then the level of overlap with the DNA mass was quantitatively analyzed. The results showed that regions associated with ongoing transcription were de-condensed and extended from fibrous structures.