Combined evaluation of lymph node metastasis in squamous cell carcinoma of the oral cavity

e -钙粘蛋白和/或连环蛋白的异常表达与多种癌症类型的侵袭性和转移有关。然而，E-cadherin和catenins的表达是维持还是减少，其组织分布尚不清楚。此外，其临床意义尚未在舌鳞状细胞癌（SCC）中进行研究。采用单克隆抗体免疫组化方法，对79例舌鳞状细胞癌经福尔马林固定、石蜡包埋的活检标本进行E-cadherin和catenins在肿瘤浅表、中部和外周侵袭前部的膜表达进行了评价。E-cadherin和α-、β-、γ-catenins的膜性表达在肿瘤浅表至中部较外周侵前部更为保留（P值分别为0.0007、0.0001、<0.0001、<0.0001）。单因素分析显示，E-cadherin、β-catenin和γ-catenin侵袭前膜表达与肿瘤分期呈显著负相关（P分别为0.02、=0.02和=0.03），E-cadherin和γ-catenin膜表达降低与肿瘤分期呈显著负相关（P分别为0.01和=0.04）。此外，E-cadherin、α-catenin、β-catenin、γ-catenin的膜内表达降低也与癌浸润程度（P分别为0.008、0.01、0.02、0.02）和病理证实的淋巴结转移程度（P分别为0.0001、0.007、0.02、0.001）显著相关。多因素logistic回归模型显示，E-cadherin和/或γ-catenin膜表达的减少或丧失是颈淋巴结转移的独立预测因子（相对危险度；13.5）。这些结果表明，评估肿瘤外周侵袭前E-cadherin和γ-catenin膜表达在临床上是有用的，特别是对于选择需要早期颈部治疗的舌SCC患者。少

Aberrant expression of E-cadherin and/or catenins has been associated with invasiveness and metastasis in a variety of cancer types. However, tissue distribution of presrve or reduce of E-cadherin and catenins expression are unclear. Its clinical significance, moreover, has not been investigated in squamous cell carcinoma (SCC) of the tongue. Membranous expression of E-cadherin and catenins at superficial to middle part and peripheral invading front of the tumor were evaluated in formalin fixed, paraffin embedded biopsied specimens from a series of 79 SCC of the tongue by immunohistochemical methods using monoclonal antibodies. E-cadherin and α-, β-, and γ-catenins membranous expression were more preserved in superficial to middle part of the tumor than those of peripheral invading front (P=0.0007, =0.0001, <0.0001, and<0.0001, respectively). Univariate analysis revealed that membranous expression of E-cadherin, β-catenin, and γ-catenin at the invading front of the tumor showed siginif … More icantly reverse correlation with the T stage (P=0.02, =0.02, and =0.03, respectively), and reduced membranous expression of E-cadherin and γ-catenin was correlated with the TNM stage (P=0.01 and =0.04, respectively). In addition, reduced membarnous expression of E-cadherin and α-catenin, β-catenin, and γ-catenin also correlated significantly with the grade of carcinoma invasion (P=0.008, =0.01, =0.02, and =0.02, respectively) and pathologically proven lymph node metastasis (P<0.0001, =0.007, =0.02, and =0.001, respectively). Multivariate logistic regression models demonstrated that the combined evaluation of reduction or loss of E-cadherin and/or γ-catenin membranous expression was the independent predictor of cervical lymph node metastasis (relative risk ; 13.5). These results indicated that the assessment of E-cadherin and γ-catenin membranous expression at the peripheral invading front of the tumor is clinically useful, especially for selecting patients who required early neck treatment in SCC of the tongue. Less