Development of artificial tubes to be used for repairing a large peripheral nerve gap

首先，我们建立了去除骨骼肌纤维中细胞成分后基底层恢复的方法：新鲜的肌肉组织用洗涤剂(3%十二烷基硫酸钠，2天37℃)处理。这种经过洗涤剂处理的肌肉组织被移植到大鼠的坐骨神经中。移植后2周可见大量再生纤维，移植后4周可见大量再生纤维伸入远端残端。在此基础上，将5~6 cm长的移植物移植到兔坐骨神经内。移植4周后，大量再生纤维伸入远端残端。电生理显示胫前肌在运动终板和肌梭处均有神经支配，用L-乳酸-己内酯管覆盖移植物是有效的神经再生方法。其次，利用人羊膜细胞外基质作为移植物的可能性是EXA…。更多的地雷。人羊膜用0.01%无花果酶处理，去除上皮细胞，制成薄片(ACS)，保存在无菌条件下(0.1%苯扎溴铵)。由这些薄板制成的管子基本上有三种：(A)管子直径1~2 mm，壁厚7~10层，(B)管束直径0.2 mm，(C)管子直径1~2 mm，外加地塞网覆盖。将这些直径为1 cm的管子移植到大鼠坐骨神经上，于移植后2~4周进行检查。移植物(A)神经再生良好，而(B)、(C)神经再生不佳。电生理检查显示，移植后9个月，胫前肌有良好的神经支配。这表明，如果制作得当，ACS是一种很有前途的周围神经再生的移植材料。制备胶原基质的方法可以在其上完全修复基底层，以及在潮湿条件下将ACS卷成管的方法还需要进一步的研究。较少

First of all, we have developed the method by which the basal laminae can be restored after cellular components had been removed from the skeletal muscle fiber : the fresh muscle tissue was treated by detergent(3% sodium dodecyl sulfate, 2 days 37 ℃). Such detergent-treated muscle tissues were grafted into the rat sciatic nerve. Many regenerating fibers were observed 2 weeks after grafting, and greatly increased in number, extending into the distal stump 4 weeks after grafting. Then, based on these finding, long grafts(5〜6 cm)were transplanted into the rabbit sciatic nerve. Four weeks after grafting, numerous regenerating fibers extended into the distal stump. Electrophysiology demonstrated that tibialis anterior muscle was reinnervated both at motor endplates and at muscle spindles.The covering of the graft by L-lactic acid caprolacton tube was recognized to be effective for nerve regeneration.Next, the possibility of utilizing the extracellular matrix of human amnion as graft was exa … More mined. The human amnion was treated by 0.01% ficin for removing the epithelial cells, and manufactured into a thin sheet(ACS), which were stored in a sterile condition(0.1% benzalkonium). From these sheets, basically three different types of tubes were manufactured ; (a)tubes 1〜2 mm in diameter, and 7〜10 layers in wall, (b)bundles of thin tubes in 0.2 mm in diameter each, (c)tubes 1〜2mm in diameter with the additional covering by dexon mesh. These tubes(1 cm) were grafted to the rat sciatic nerve, and examined 2〜4 weeks after grafting. Good nerve regeneration was observed in the graft(a), but not in(b)and(c). Electrophysiology showed the good reinnervation occurred in the anterior tibialis muscle 9 months after grafting. This indicates that ACS, when properly manufactured, is a promising material as the graft for the peripheral nerve regeneration.The further study is needed to develop the method by which the collagen matrix is prepared on which the basal laminae can be fully restored, and the way in which the ACS can be rolled into tubes in a wet condition. Less