Epidemiology on piroplasmosis of demestic animals in Mongolia

家畜巴贝斯虫病在世界大部分地区造成巨大的经济损失。在蒙古，在马中观察到巴贝斯虫病的高流行率，但在牛中尚不清楚。本研究采用体外培养法分离了蒙古马巴贝斯虫，并对分离的巴贝斯虫进行了遗传分析。对牛巴贝斯虫抗体和DNA片段的检测也进行了分析。马巴贝斯虫的抗原分析和抗体检测马巴贝斯虫重组蛋白(MEA-1)和卡巴氏巴贝斯虫(BC48)重组蛋白可作为血清诊断的抗原，如酶联免疫吸附试验或乳胶凝集试验。马分枝杆菌和卡巴利分枝杆菌在蒙古中部的马中都是地方病，为81。IFAT检测的110个样本中，8%的马对这两种感染都呈阳性。用GST-BC48对采自蒙古中部两个不同地区的209份马血清进行了酶联免疫吸附试验。2种不同AR-…人群中卡巴利球虫感染情况高EAS分别为90.3%和23.4%。体外培养分离马巴贝斯虫40份血样中检出马巴贝斯虫33份。对EMA-1和EMA-2的DNA序列分析表明，分离株之间具有较高的遗传同源性，蒙古国分离株与美国农业部分离株在核苷酸和氨基酸序列上具有较高的同源性。利用聚合酶链式反应技术，在所有样本和54份样本中的7份样本中分别检测到马巴贝斯虫EMA-1基因和卡氏巴贝斯虫BC48基因。制备重组牛巴贝斯虫杆状病毒相关蛋白-1(RAP-1)，并将其作为抗原用于酶联免疫吸附试验。该方法能区分牛分枝杆菌感染血清、双链杆菌感染血清和正常牛血清。结果表明，在高倍体昆虫细胞中表达的重组RAP-1有可能成为检测牛巴氏杆菌抗体的有效抗原。较少

Babesiosis in domestic animals causes enormous economic losses throughout most of the world. In Mongolia, high prevalence of babesiosis was observed in horses but not known in cattle. In the present study, in vitro cultivation was used for isolation of Babesia parasites from Mongolian horses and geneticanalysis of isolated parasites has done. Detection of antibody and DMA fragments of bovine Babesia parasites were also analyzed.1. Antigen analysis of equine Babesia parasites and survey of antibodiesRecombinant proteins of B. equi (MEA-1) and B. caballi (BC48) were found to be useful as antigens for serodiagnostic methods such as ELISA or latex agglutination test. Both B. equi and B. caballi are endemic in horses in Central Mongolia as 81. 8% of horses were seropositive to both infections in 110 samples by IFAT. A total of 209 horse sera obtained from 2 different areas of Central Mongolia were examined by ELISA using GST-BC48. The prevalence of B. caballi infection in the 2 different ar … More eas was 90.3 % and 23.4 %.2. Isolation of equine Babesia parasites by in vitro cultivation.B. equi parasites were found in 33 samples from 40 blood samples. Analysis of DNA sequence of EMA-1 and EMA-2 suggests that B. equi has high genetic homology among isolates and that B. equi isolated in Mongolia has high homology in nucleotide and amino acid sequences with USDA strain.3. Survey on ticks transmittable Babesia parasitesEMA-1 gene of B. equi and BC48 gene of B. caballi were detected in all samples and seven of the 54 samples of Dermacenor nuttalli adult ticks by PCR, respectively.4. Survey on bovine babesiosisRecombinant rhoptry associated protein-1 (RAP-1) of Babesia bovis was produced and i used as antigen in ELISA. The ELISA was able to differentiate between B. bovis-infected sera and B. bigemina-infected sera or noninfected normal bovine sera. The results demonstrate that the recombinant RAP-1 expressed in high five insect cells might be a useful antigen for detection of antibodies to B. bovis. Less