Biomedical approach of new materials with well-organized interfaces

1)本研究采用嵌段共聚物制备纳米结构。特别是，在表面上构建功能化聚乙二醇（PEG）层的新方法受到关注，以通过PEG共轭配体实现靶蛋白的特异性吸附，同时最小化对其他蛋白质的非特异性吸附。此外，从制备具有独特大分子结构的新型功能表面涂层的角度，研究了具有交联核的嵌段共聚物胶束的表面组织。胶束附着表面和层状胶束形成的薄水凝胶层具有不结垢的特性，可作为疏水试剂的储层。这些以刷状或胶束状的peg功能化表面可用于医学和生物学的各个领域，以构建高性能的医疗设备，包括组织工程的支架和药物输送系统的基质。近年来，越来越多的载体系统因其高载药能力和在体内独特的处置特性在药物靶向领域受到越来越多的关注。本研究项目旨在利用嵌段共聚物的多分子组装形成的聚合物胶束作为新型核-壳型胶体载体用于药物和基因靶向。基于组份嵌段共聚物的疏水相互作用、静电相互作用、金属络合作用和氢键等驱动因素的差异，详细研究了水环境下胶束化过程。嵌入在亲水性栅栏中的分离核被证明是基因、酶和具有不同特征的各种药物的储存库。从设计用于受体介导的药物传递的胶束载体系统的角度出发，研究了聚合物胶束外表面功能化以改变其物理化学和生物学特性。此外，研究人员还研究了聚合物胶束的体内分布，证明了它们的长循环特性和显著的肿瘤蓄积，强调了它们在肿瘤靶向治疗中的应用前景。作为高分子胶束载体体系的一个重要研究方向，其作为非病毒基因载体的可行性也得到了验证。少

1) Nanostructure fabrication from block copolymers was carried out in this study. Particularly, novel approaches for the construction of functionalized poly(ethylene glycol) (PEG) layers on surfaces were focused to attain the specific adsorption of a target protein through a PEG-conjugated ligands with a minimal non-specific adsorption of other proteins. Further, surface organization of block copolymer micelles with cross-linking core was studied from a standpoint of the preparation of a new functional surface-coating with a unique macromolecular architecture. The micelle-attached surface and the thin hydrogel layer made by layered micelles exhibited nonfouling properties and worked as the reservoir for hydrophobic reagents. These PEG-functionalized surface in brush form or in micelle form can be used in diverse fields of medicine and biology to construct high-performance medical devices including scaffolds for tissue engineering and matrices for drug delivery systems.2) Recently, coll … More oidal carrier systems have been receiving much attention in the field of drug targeting because of their high loading capacity for drugs as well as of their unique disposition characteristics in the body. This research project was aimed at the utility of polymeric micelles formed through the multimolecular assembly of block copolymers as novel core-shell typed colloidal carriers for drug and gene targeting. The process of micellization in aqueous milieu is studied in detail based on the differences in the driving force of core segregation, including hydrophobic interaction, electrostatic interaction, metal complexation, and hydrogen bonding of constituent block copolymers. The segregated core embedded in the hydrophilic palisade is shown to function as a reservoir for genes, enzymes, and a variety of drugs with diverse characteristics. Functionalization of the outer surface of the polymeric micelle to modify its physicochemical and biological properties is investigated from the standpoint of designing micellar carrier systems for receptor-mediated drug delivery. Further, the body distribution of polymeric micelles is studied to demonstrate their long-circulating characteristics and significant tumor accumulation, emphasizing their promising utility in tumortargeting therapy. As an important perspective on carrier systems based on polymeric micelles, their feasibility as nonviral gene vectors is also demonstrated in this study. Less