Liquid ventilation as a bridging to lung transplantation

没有适用的通气装置来支持慢性呼吸衰竭患者。在本研究项目中，我们试图评估是否液体通气使用通过肠道和/或腹膜将增加氧合的功能。我们不仅在细胞实验中使用FC-77，而且在麻醉大鼠模型中使用FC-77。在细胞实验中，我们将氧合的FC-77与人外周静脉血在试管中混合。我们发现在混合后的第一分钟PaO 2显著增加，PaCO 2降低。我们还发现FC-77能够在transwell插入系统中穿过人肺动脉内皮细胞和人肺泡上皮细胞（A549）的单层递送O2和吸收CO2。在大鼠实验中，大鼠麻醉后，以低潮气量（VT= 5 ml/kg）和低通气速率（f=20）通气，然后将氧合的FC-77（20 ml/kg）导入结肠或腹腔。我们发现，在FC化合物给药后4小时内，结肠和/或腹膜实验中的PaO 2逐渐增加。然而，腹膜给药似乎不仅在氧合方面，而且在去除CO2的能力方面都稍有上级。我们认为FC-77可用于慢性呼吸衰竭患者的抢救。我们在这个项目中获得的数据表明，在不久的将来，腹膜液体通气可能成为可能。

There has been no applicable ventilation devises to support patients with chronic with respiratory failure. In the present research project, we sought to evaluate whether liquid ventilation using across gut and/or peritoneum would be function to increase oxygenation. We utilized FC-77 in not only cell experiments, but also anesthetized rat models. In cell experiments, we mixed oxygenated FC-77 with human peripheral venous blood in a test tube. We found a marked increase in Pao2 and a decrease in PaCO2 in the first minute following mixture. We also found that FC-77 was able to deliver O2 and absorb CO2 across the monolayers of human pulmonary arterial endothelial cells and human alveolar epithelial cells (A549) in the transwell insert systems. In rat experiments, rats were anesthetized and ventilated with low tidal volume (VT=5ml/kg) at low ventilation rate (f=20), and then oxygenated FC-77 (20ml/kg) was introduced into colon or peritoneal cavity. We found a progressive increase in PaO2 in both colon and or peritoneum experiments over 4 hours following administration of FC compound. However, peritoneal administration seemed to be somewhat superior not only in oxygenation, but also in the ability of removing C02. We believe that FC-77 would be applicable in the rescue of patients with chronic with respiratory failure. Our data obtained in this project indicate that liquid ventilation across the peritoneum would potentially become possible in near future.