Expression of enamel matrix protein in vitro

一些研究已经检查了牙釉质基质蛋白在牙根形成和牙周再生中的作用，尽管这些研究大多集中在一些先前涉及的特定基因上。然而，最近在表达谱分析方面的进展使得检查这些过程中涉及的遗传反应的范围成为可能。因此，在本实验中，我们利用该技术在体外测定牙釉质基质蛋白对牙周膜细胞基因活性的影响。与对照细胞相比，这些细胞的RNA合成水平有所提高。

Several studies have examined the role of enamel matrix proteins in root formation and periodontal regeneration, although most of these have focused on a few specific genes which had previously been implicated. However, recent advances in expressional profiling have made it possible to examine the range of genetic responses involved in these processes. In the present experiments, we have therefore utilized this technique to determine the effects of enamel matrix proteins on the gene activities of periodontal ligament cells in vitro. Such cells were found to have an elevated level of RNA synthesis compared with control cells.