Study of the relation between the modulations of allergic response and the sick house syndrome caused by indoor air pollutants

众所周知，免疫学机制与病态建筑物（房屋）综合征（SBS，SHS）的发生有关，因为在SBS患者中经常观察到与变态反应相关的症状。在此基础上，我们将引起SBS的主要室内空气污染物甲醛（HCHO）暴露于小鼠（B6C3F1，雌性），通过观察HCHO对小鼠免疫反应的调节作用和实验诱导的过敏反应来评价HCHO的健康效应。我们还调查了SBS患者在实验性气态HCHO暴露小鼠中报告的健康影响，而没有任何免疫治疗。此外，我们还调查了通过消化道接触甲醛对健康的影响。通过给予HCHO处理的动物饲料颗粒或添加HCHO的饮用水使小鼠暴露于HCHO。对照组给予纯饲料和饮用纯净水。500ppb气态甲醛暴露增强绵羊抗HCHO抗体形成反应 ...更多信息 红细胞和淋巴细胞对有丝分裂原刺激的增殖反应。在致敏和诱导症状两个阶段均观察到速发型过敏反应的增强调制。甲醛染毒小鼠外周血淋巴细胞DNA非整倍体的出现，是部分SBS患者的特征性表现之一。HCHO染毒小鼠海马和嗅球催乳素mRNA表达明显增加。经甲醛处理的饲料摄入显著降低了消化道细菌数量的剂量依赖性，而相同数量的甲醛通过饮用水摄入的效果较弱。腹腔注射脂多糖诱导的肝脏细胞因子应答的抑制，该现象在消化道细菌数量减少的小鼠中观察到，在HCHO处理的饮食喂养小鼠中也观察到。结果表明，吸入或口服甲醛均可引起机体免疫反应和变态反应的改变，并出现SBS病例的特征性现象。提示免疫功能在SBS的发生发展中起重要作用。少

It is well known that the immunological mechanism concerns in developing the sick building (house) syndrome (SBS,SHS), since allergy relating symptoms are frequently observed in the SBS patients. At the standing point of those findings, we exposed gaseous formaldehyde (HCHO) which is the major indoor air pollutant to cause SBS to mice (B6C3F1,female) and evaluated the health effects of HCHO by observing the modulations of the immune response and the experimentally induced allergic reactions. And we also surveyed the health effects which are reported in the SBS patients in the experimentally gaseous HCHO exposed mice without any immunological treatment. Furthermore we investigated the health effects of HCHO exposure via alimentary tract. Mice were exposed to HCHO by giving of HCHO treated animal diet pellets or HCHO added drinking water. For control group, intact diet and pure drinking water were given. Exposure of 500ppb gaseous HCHO enhanced the antibody forming response against sheep … More red blood cells and the lymphocyte proliferating response by mitogen stimuli. Enhancive modulations of immediate type allergic reaction were observed in both phases of sensitization and induction of symptoms. Aneuploid DNA in peripheral lymphocyte which is one of characteristic sign observed in some case of SBS patients was detected in gaseous HCHO exposed mice. Induction of prolactin mRNA in hippocampus and olfactory bulb was obvious in HCHO exposed mice. Ingestion of HCHO treated diet dramatically reduced the number of bacteria in alimentary tract dose dependency, whereas same quantity of HCHO via drinking water did weakly. Suppression of cytokine response in liver induced by i.p.of lipopolysaccharide, that phenomenon is observed in the mice with reduced number of alimentary tract bacteria, also observed in HCHO treated diet fed mice. The results indicate that both routes of HCHO exposure, inhalation or per oral, induce the modulation of immune response and allergic reactions as well as characteristic phenomena to SBS cases. It may suggest that immune function play some significant role in the development of SBS. Less