Study on health disorders by mix exposure of arsenic and fluoride in China via drinking water

在内蒙古的野外调查中，检测到井水中砷（As）和氟（F）的浓度较高。尿砷和氟浓度显示，居民暴露于这两个元素同时饮用井水。其中有砷中毒和氟斑牙相关的皮肤病。两种症状均以男性为主。在动物实验中，将C57 BL/6 n小鼠暴露于砷（10 ppm）、氟（1或5 ppm）或砷+氟中50或100天。氟、砷染毒50天的雌性小鼠股骨骨面积大于对照组，而染毒100天的雌性小鼠股骨骨面积与对照组相比无显著性差异。从第50天开始，男女股骨骨密度均呈高值。从50天开始，雌雄小鼠股骨强度与对照组无差异，但在100天，雄性F组或As+F组和雌性F组表现出较弱的趋势。结果表明，砷、氟对骨生长、骨密度和/或骨强度的影响存在性别差异。血清GM-CSF在两种性别中均未显示任何趋势。虽然氟对骨骼的影响是已知的，但我们的研究结果首次在动物实验中证明砷暴露对骨骼有影响。在内蒙古地区饮用砷污染井水慢性砷中毒的现场调查中，骨健康检查是必须考虑的。

In the field research in Inner Mongolia, high concentration of arsenic (As) and fluoride (F) in well were detected. Urine As and F concentrations revealed that residents were exposed to both elements simultaneously by drinking well water. Skin disorders associated to As and dental fluorosis was diagnosed in them. Both symptoms were dominant in male. In animal study, C57BL/6n mice were exposed to As (10ppm), F (1 or 5ppm) or As+F for 50 or 100 days. Area of femur bone from 50 days F or As exposed female mice were larger than control, but not from 100 days exposed groups. Bone densities of femur bone from 50 days As exposed both genders showed high. Strength of femur bone from 50 days As exposed both gender mice had no difference between control, but at the exposure day 100, male F or As+F and female F groups showed weaker tendencies. As the results, As and F influenced to bone growth and density and/or strength with gender difference. Serum GM-CSF did not shows any tendencies in both genders. Although it is known that F affect bone, out results showed the first evidence that As exposure affected bone in animal study. Health check on bone have to consider in the field research for chronic arsenic poisoning by drinking As contaminated well water in Inner Mongolia.