Developmental research of biochemical test based on the molecular biological analysis of sick house syndrome

基于病屋综合征分子生物学分析的生化检测进展研究

• 批准号: 16390167
• 负责人: YOSHIDA Takahiko
• 金额: $ 9.09万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390167/
• 关键词: sick house syndrome; multiple chemical sensitivity; peripheral blood mono nucleocyte; DNA micro alley analysis; marker gene; mRNA; biomarker; bio chemical analysis

There is no significant biochemical test for multiple chemical sensitivity (MCS). Practicable medical facilities for diagnosis of MCS are limited since a clean room is necessary to confirm the causative agent. The aim of this research is the establishment of the diagnostic test for MCS using biochemical test of peripheral blood samples. Sixteen patients diagnosed as MCS, 9 healthy subjects and 4 subjects for control were enrolled in this research. Pooled total RNA obtained from peripheral blood mononuclear cells (PBMCs) of control subjects and similarly prepared total RNA from each MCS patient or healthy subject were labeled with different color fluorescence respectively, and thoroughly explored the marker gene by DNA micro alley analysis. The gene with over than 2 times or less than half expression in the MCS patients in contrast with no change in the healthy subjects was judged as a varied gene. Although there was no gene which varied in all MCS patients, 28 genes (increase:2,decreas … More e:26) were detected as varied genes among over half MCS patients. Ten distinguished genes were identified in the analysis between the control and MCS patients whose gene expression level was high. Five genes of them were also identified as decreasingly varied genes in PBMCs or spleen cells from the mouse which were exposed to formaldehyde (HCHO). Those genes were possibly to be diagnostic marker genes, since they were thought to be varied by stress, although the correlation between those genes and the occurrence of MCS remained unclear. We considered the possibility to detect the proteins as gene products in serum. But those products were enzymes or receptors expressed in tissue or cell and unable to detect in blood, or cytokines which released into blood at too low level for measuring. It is failed to be established the general biochemical test for MCS diagnosis in this study. Further researches using the protein chip methods are required to focus the biomarker produced and poured into blood by liver, kidney or nerves systems. Less

目前还没有针对多重化学敏感性(MCS)的显着生化检测。诊断MCS的实用医疗设施有限，因为必须有洁净室来确认病原体。本研究的目的是建立外周血生化试验诊断MCS的方法。本研究共纳入16例MCS患者、9例健康人和4例正常对照。分别用不同颜色的荧光标记对照组外周血单个核细胞(PBMCs)的总RNA和同样制备的MCS患者或健康人的总RNA，并通过DNA微巷分析对标记基因进行深入的分析。在MCS患者中表达超过2倍或不到一半的基因被认为是变异基因，而在健康人中没有变化。虽然在所有MCS患者中没有发生变化的基因，但有28个基因(增加：2，减少…在半数以上的MCS患者中检测到更多的e：26)变异基因。在对对照组和MCS患者的分析中，发现了10个基因表达水平较高的差异基因。其中5个基因也被鉴定为甲醛(HCHO)暴露小鼠外周血单个核细胞(PBMC)或脾细胞中差异减少的基因。这些基因可能是诊断标记基因，因为它们被认为随着压力而发生变化，尽管这些基因与MCS的发生之间的相关性尚不清楚。我们考虑了将这些蛋白质作为基因产物检测到血清中的可能性。但这些产品是在组织或细胞中表达的酶或受体，在血液中无法检测到，或者是释放到血液中的细胞因子水平太低，无法检测。本研究未能建立MCS诊断的通用生化检测方法。使用蛋白质芯片方法的进一步研究需要关注由肝脏、肾脏或神经系统产生并注入血液的生物标记物。较少