Study on detection of urinary spermine in cadaver

在我们的兴奋剂药物测试经验中，我们发现检测多胺。尿中检测到的精胺在细胞生长中起着非常重要的作用，偶尔在相当高的水平引起了我们的兴趣，如果精胺的释放影响任何死亡途径。在这项研究中，一个快速的和。建立了尿多胺的气相色谱/质谱（GC/MS）准确定量分析方法。采用高效液相色谱-柱后衍生-荧光检测（HPLC）和气相色谱/质谱法（GC/MS）对法医学尸体和急危医院患者的尿精胺浓度进行了测定。HPLC采用市售的氨基酸分析系统，GC/MS采用我们的安非他明法进行修饰，其中提取衍生化（七氟丁基化）通过充满硅藻土的提取管进行。男性尸体的尿精胺平均浓度明显高于女性尸体。尸体与患者尿精胺水平比较，尸体水平相对较高。为方便起见，阳性标准的阈值水平设置为尸体80 nmol/mL和患者20 nmol/mL。尿精胺高分仅适用于有心脏疾病、脑损伤和肝损伤的男性尸体，主要适用于因心功能障碍、脑损伤等引起的成人呼吸骤停综合征的男性患者。这些结果表明，人在死亡前释放精胺有一定的机制。

In our experience of stimulant drug test, we found detecting polyamines. Urinary detection of spermine, which plays very important roles in cell growth, occasionally in considerably high level attracted in our interest if the release of spermine affected any pathway of death. In this study, a rapid and. accurate quantitative analytical method for urinary polyamines was developed using gas chromatography/mass spectrometry (GC/MS). By using high performance liquid chromatography with post-column derivatization on fluorescence detection (HPLC) and GC/MS, urinary spermine concentrations of cadavers in forensic medicine and patients in emergency and critical care hospital were measured for preliminary study. HPLC was performed by using amino acid analytical system commercially provided, and GC/MS was developed modifying with our amphetamine assay in which an extractive derivatization (heptafluorobutyrylation) was carried out in passing through an extracting tube filled with diatomaceous earth. Average of urinary spermine concentrations were considerably higher in male cadavers than in female ones. In comparison of cadavers and patients in urinary spermine levels, cadaver levels were relatively higher. Threshold levels for positive criterion were, for convenience sake, set at 80 nmol/mL for cadavers and-at 20 nmol/mL for patients. High scores of urinary spermine were only indicated in male cadavers with heart diseases, brain injuries and liver injuries, and in male patients mainly with adult arrest respiratory disease syndrome due to cardiac dysfunction, brain injuries, etc. These results suggest that there are some mechanisms in man releasing spermine before death.