Transplantation of stem cells from sympathetic ganglion into the brain Parkinson disease

干细胞具有多能性和自我更新能力，是理想的移植供体来源。我们研究了将交感神经节移植到帕金森病患者的大脑中，显示出对帕金森病患者运动迟缓和肌肉僵硬等症状的效果。在目前的研究项目中，我们应用干细胞移植治疗帕金森病，并检测了移植对帕金森症状的影响。我们还对帕金森患者进行了交感神经节脑内移植的临床研究。将Nestin-GFP(绿色荧光蛋白)小鼠的神经干细胞移植到6-OHDA注射的帕金森病动物模型大鼠的脑内。移植的干细胞在宿主大脑中存活良好。他们增加了细胞数量，并延长了宿主大脑中的轴突。存活的细胞在宿主脑中表达免疫反应。本试验的成活率为6-10%。移植大鼠给予苯丙胺后旋转评分显著降低(平均77.3%)。对4例接受胸交感神经节治疗的患者进行了长期的仔细观察，结果显示非交感神经节的时间百分比显著减少。体外培养的神经节细胞呈TH、VMAT和AADC免疫反应。本研究在帕金森病动物模型和帕金森病患者模型上清楚地证明了干细胞移植对症状的影响。

Stem cell has multipotent and self renewal ability, which is ideal source of donor for transplantation. We have studied transplantation of sympathetic ganglion into the brain with Parkinson disease, showing effect on parkinsonian symptoms such as bradykinesia and muscle rigidity. In the present research project we have applied transplantation of stem cells for Parkinson disease and examined effect of transplantation on parkinsonian symptoms. We also studied clinical research of sympathetic ganglion transplantation in the brain with Parkinson patients. Neural stem cells from nestin-GFP(green fluorescence protein) mouse were transplanted into the brain of 6-OHDA injected rat(an animal model of Parkinson disease). Transplanted stem cells well survived in the host brain. They increased cell numbers and extended their axons in the host brain. Surviving cells expressed TH imnmnoreactivity in the host brain. Survival rate in this experiment were 6-10%. Rotational scores after amphetamine administration were significantly reduced (77.3% on average) in the transplanted rats. Four patients undergoing thoracic sympathetic ganglia were carefully observed for long period, showing significant reduction in the percentage of time spent in the off phase. Piece of ganglion cultured in vitro revealed TH-, VMAT- and AADC- immunoreactivity.This research project clearly demonstrated the effects of stem cell transplantation on symptoms in animal model of Parkinson disease and parkinsonian patients.