Study on the development of real-time and in vivo measurement technique of gene expression on mice using optical methods.

我们研究了发生在小鼠体内的基因表达监测技术。光学方法来解决作为基因报告或外源管理的荧光标记物表达的荧光团的分布进行了研究。为了检测具有空间分辨率的荧光，我们应用了相干检测成像技术，并且还研究了应用荧光寿命成像和光谱成像技术来消除自发荧光，相干检测成像技术提供了光学检测和天线的宽动态范围特性，以提取混浊介质中的直接向前散射光。我们将该技术应用于生物组织中的荧光检测，并实现了嵌入组织中的荧光团的可视化，深度可达20 mm。我们的结论是，这种技术的潜在用途，以确定基因表达来源于荧光蛋白在体内，这种技术也可以适用于各种生物传感使用荧光标记技术。

We have studied the monitoring technique of gene expression occurred in the internal body of mice. Optical methods to resolve the distribution of fluorophors expressed as gene reporters or exogenously administered fluorescent markers are investigated. In order to detect the fluorescence with spatial resolution, we have applied the coherent detection imaging technique, and also examined to apply a fluorescence life-time imaging and a spectral imaging technique to eliminate the auto-fluorescence, which is the background emission of tissue being a limiting factor to determine the minimum detectable signal The coherent detection imaging technique offers the wide dynamic range of optical detection and antenna properties to extract the straight forward scattering light in turbid media. We have combined this technique to apply for the detection of fluorescence in the biological tissue, and achieved the visualization of fluorophores embedded in the tissue at the depth up to 20mm from the surface. We conclude the potential usefulness of this technique to identify gene expressions originating from fluorescent proteins in vivo and this technique can also be applicable for a variety of biological sensing using fluorescent labeling technique.