Absorption Kinetics of the Main Allergen in Egg White on the Intestinal Mucous Membrane of Infants

本研究从以下几个方面进行了研究：1.过敏大鼠(幼年)通过肠粘膜(IMM)进入蛋清(蛋白)的情况2.食物过敏后IMM的情况3.过敏大鼠给予过敏原食物诱导免疫耐受获得以下结果：1.幼年大鼠过敏后通过IMM的过敏原明显多于过敏前。此外，在过敏发生后两周，通过IMM的过敏原的数量明显多于紧接之后的量。因此，过敏反应在过敏发展的1-2周内被传导到IMM。2.过敏食物给药22天后，皮肤出现变态反应炎症。佩尔的IMM上的斑块扩张和肿胀。变应原持续给药组大鼠空肠组织中肥大细胞数量明显增加，杯状细胞和绒毛小泡解体。3.在免疫耐受方面，持续给药后IL-4和干扰素-γ水平分别降低和升高。在间断给药组，在已经停止给药的情况下，IL-4水平在给药第二周升高。当给药后不久停止给药时，脾细胞中的T细胞主要分化并增殖为Th2细胞。但当恢复给药时，T细胞向Th1细胞的分化被诱导，并观察到总IgE或OM IgE抗体单位的显著下降，提示诱导免疫耐受。因此，口服适当剂量的过敏原不会引起过敏，建议在变态反应治疗中有效，因为其免疫或免疫反应降低。

This study investigated the following aspects :1.Passage of allergens into the egg white (protein) via the intestinal mucous membrane (IMM) in allergic rats (considered as infants)2.Condition of the IMM after development of food allergy3.Immune tolerance induction by the administration of allergen foods in allergic ratsThe following results were obtained :1.In juvenile rats, a significantly larger amount of allergens passed via the IMM after development of allergy than before. Moreover, the amount of allergens passing via the IMM two weeks after allergy development was significantly larger than that immediately after. Therefore, the allergic effect was mediated to the IMM within one-two weeks of allergy development.2.Allergic inflammation was observed on the skin 22 days after the administration of allergic food. Peyer's patches on the IMM were expanded and swollen. Disintegration of goblet cells and villous blebs was observed in the allergen administration group ; the mast cell number increased significantly in the jejunum tissues.3.Regarding immune tolerance, the IL-4 and IFN-γ levels decreased and increased, respectively, after continuous allergen administration. In the discontinuous group, the IL-4 level increased in the second week of allergen administration when administration had already been discontinued. When the allergen administration was discontinued shortly after its administration, the T-cells in the splenic cells mainly differentiated and proliferated into Th2 cells.However, when allergen administration was resumed, differentiation of T cells into Th1 cells was induced, and a significant decrease was observed in the total IgE or OM IgE antibody units, suggesting the induction of immune tolerance.Here, oral administration of the allergen in an appropriate dose that did not cause allergy was suggested to be effective in allergy treatment because of its decreased immunization or immune response.