Self-assembly of the Supramolecular Complexes of Chitosan-Nanoparticles Hybrid Composites with Iodine and Their Biological activities

迄今为止，我们已经获得了具有多种功能的天然阳离子多糖壳聚糖（Ch）与碘络合诱导的自组装的研究结果。为了开发具有生物活性的新型可持续材料，合成了壳聚糖与聚丙烯亚胺树状大分子（PPID）、富勒烯C60、金颗粒等纳米粒子的杂化化合物。然后，尝试用碘络合诱导每个杂化体的自组装超分子。结果表明，Ch与PPID （CDH）杂交后，CDH与碘配合物（CDHI）的理化性质取决于PPID的生成，其中G1代的络合能力最强。无论PPID世代如何，cdh的抑菌活性均低于Ch。另一方面，CDHI复合物对大肠杆菌（E.coli）的抑菌活性受到碘的影响。在此基础上，以四乙二醇为间隔剂，合成了Ch与C60单加合物的共轭物（CFH）。ESR测量结果表明，CFH具有清除自由基的生物活性。此外，将金胶体膜浸泡在硫代壳聚糖溶液中24h，制备了壳聚糖自组装单层（SAM）膜。在壳聚糖功能化云母上通过静电相互作用制备了新型的Ch或ChI配合物与Au粒子自组装的多层膜。自组装的单层和多层Ch或ChI配合物显示出抗菌活性。

We have so far obtained the findings that natural cationic polysaccharide, chitosan (Ch), with multifunctionalities assumes the self-assembly induced by the complexation with iodine. In order to develop novel sustainable materials with biological activities, hybrid compounds of chitosan with some nanoparticles, such as polypropyleneimine dendrimer (PPID), fullerene C60, and gold particle, were synthesized. Then, self-assembled supramolecule for each of the hybrids was attempted to be induced by the complexation with iodine. As a result for the hybrid of Ch with PPID (CDH), the physicochemical properties of the complexes of CDH with iodine (CDHI) were found to be depended on the generation of PPID, whose G1 generation had the greatest complexation ability. The CDHs had the antimicrobial activities, which were lower than Ch, regardless of the PPID generations. On the other hand, the antimicrobial activities of the CDHI complexes against Escherichia coli (E.coli) were subjected to iodine. Furthermore, we have synthesized the conjugate (CFH) composed of Ch and monoadduct C60 with tetraethylene glycol as spacer. The ESR measurements revealed that the CFH had a biological activity as radical scavenge. In addition, a self-assembled monolayer (SAM) film of chitosan were prepared by immersing Au colloids film in thiolated chitosan solution for 24hours. Novel self-assembled multilayer films of Ch or ChI complex and Au particles were prepared through electrostatic interaction on chitosan functionalized mica. The self-assembled monolayer and multilayer of Ch or ChI complexes displayed the antimicrobial activities.