Study for neuronal regeneration in ischemic brain using bone marrow transplantation

神经元再生的概念已经从过去的再生医学迅速改变。近年来，胚胎干细胞的应用为神经元的分化和移植提供了可能。再生在临床领域非常感兴趣，脑缺血实验我们成功地建立了完全闭塞的大脑中动脉模型，通过加入PDGF-BB和/或B-β-CD，使骨髓干细胞（BMC）向神经元方向分化，包括造血细胞和间充质细胞。应用免疫细胞化学和PCR技术对去系小鼠骨髓基质细胞（BMC）中的CD_（34）CD_（34）-和CD_（34）+细胞进行分选和神经元表达的检测，发现骨髓基质细胞呈lin-CD_（34）-成体小鼠BMC向神经元分化。

The concept of neuronal regeneration has been rapidly changed from the past regenerative medicine. Recently neuronal differentiation and transplantation could be possible using by embryonal stem cell. Regeneration is much interested in the clinical field, so we studied for the possibility of neuronal regeneration in ischemic bran using bone marrow transplantation.Experiment of ischemic brainWe succeeded in modeling complete occulusion of ipsilatenal middle cerebral artery.Differentiation of bone marrow stem cells (BMC)BMS including the hematopoietic and mesenchymal cells could be differentiated into neuronal direction by adding PDGF-BB and/or b-FGF.Sorting and neuronal expression of CD34CD34- and CD34+ cells of lineage depleted BMC were sorted and neuronal differentiation showing lin-CD34-adult murine BMC were found by immunocytochemistry and PCR.Improvement of ischemic brainThe ischemic region was improved by transplantation of BMC.