The time of acquired tolerance formation in liver transplantation, and elucidation and clinical application of participating bioacctive substance

主要研究者桥本先生与藤田保健大学医学部的杉冈敦助教合作，于2004年4月开始研究。杉冈助教从他以前的研究中阐明，在使用所有小鼠品系集合的实验性肝移植中，获得性耐受是衍生的。我们从2004年6月7日开始在藤田保健大学开展肝移植项目。目前，我们已经经历了9例活体肝移植，虽然有1例23岁女性因血栓性血小板减少性紫癜去世，但其余8例均健康，其中6例儿童病例有望获得耐受。目前正在进行淋巴细胞交叉配血的研究，并对血清标本进行长期保存.临床肝移植中免疫抑制相关体液因子的研究我们最初计划用蛋白质尖端法来研究一种新的多肽。然而，很明显， ...更多信息 蛋白质针尖需要大量的费用来检查一个样本。然后，我们没有获得一个从头开始的数据，除了过去的值，直到现在。为了在门诊随访中比较未使用免疫抑制剂的病例与其他病例的免疫状态，我们选择并测量免疫相关细胞因子（IL-4，IL-8，IL-IL-10，IL-12）并对其进行评估. FK 506阻断获得性耐受的产生为了阐明小鼠肝移植后容易获得耐受的机制，阻断耐受的获得将是有用的。Sugioka教授已经证明，在小鼠肝移植中，在不给予免疫抑制剂的情况下，在任何类型的组装中都可以获得耐受。另一方面，我们知道，获得性耐受是在移植后早期严重排斥反应后产生的。因此，我们给候选人FK 506（他克莫司），这是一种有效的免疫抑制剂，通过防止初始排斥反应来阐明影响。这种治疗的结果是，我们能够防止排斥反应，但获得性耐受性的来源被阻断。少

Hashimoto who was a main researcher started study in April 2004 in cooperation with assistant professor Atsushi Sugioka in Fujita Health University School of Medicine.Assistant professor Sugioka elucidates from his previous study that acquired tolerance is derivate in an experimental liver transplantation using all assembly of mouse strains.1. We started liver transplantation program in Fujita Health University from June 7 2004. Now, we have experienced 9 cases of living donor liver transplantation.Although we lost a case of 23 year old female from thrombotic thrombocytopenic purpura, but remaining 8 cases are healthy, and acquired tolerance derivation is expected in 6 of child cases. Now we are investigating the lymphocyte cross matching and storing the serum samples with time.2. Elucidation of an immunosuppression related humoral factors in a clinical liver transplantationWe initially planed to use a protein tip method to elucidate a new peptide. However, it becomes clear that SELDI … More protein tip needs a large amount of expense to examine one specimen. We then did not obtain a denovo data concerning this procedure except only past values until the present. For a purpose to compare immune status of cases without an immunosuppressive agent with other cases in follow up at the outpatient clinic, we choose and measure immunology coherence cytokines (IL-4, IL-8, IL-IL-10, IL-12) and are evaluating it.3. A block of derivation of acquired tolerance by FK506For an elucidation of a mechanism of acquired tolerance derivation easily after mouse liver transplantation, it will be useful to block the obtaining of tolerance. Professor Sugioka has proved that the acquired tolerance is concluded in any kind of assembly without administration of immunosuppressive agent on a liver transplantation of mouse. On the other hand, we know that acquired tolerance is delivered after sever rejection in early period of the post transplantation in this liver transplantation. Therefore, we gave FK506 (tacrolimus) which was effective immunosuppressive agent to clarify influence by preventing of initial rejection, to the candidate. Resulting of this treatment, we were able to prevent the rejection, but acquired tolerance derivation was blocked. Less