A basic study for the new method of the bone formation using regeneration method for the cleft patient

首先，我们研究了唇裂和牙槽裂患者在不进行二次植骨的情况下，正畸牙移动后裂区新骨的形成情况。仅靠正畸牙齿移动不足以形成新骨。本研究的目的是研究一种无需任何供体的新骨形成。在小鼠的股骨上钻一个直径3 mm的洞。然后采用富血小板血浆移植方法进行移植。将分离的富含血小板血浆浸泡后，放置于此孔内，作为一种人工胶原。其内可见新骨形成。然后将培养的骨膜置于另一个孔内，不做任何移植，新骨形成尚不充分。为了证实钛网和胶原的临床效果，在牙槽骨上打了几个洞后，在牙槽骨上放置了用于空间形成的atelocl胶(TeleplagTM&gt；)和用于空间保持的钛网(M-^&lt；TM&gt；)。6个月后植入时，距骨胶原完全吸收，可见新生的牙槽骨。

At first we investigated about the new bone formation to the cleft area for the patient with cleft lip and alveolus using orthodontic tooth movement without the secondary bone grafting. The new bone was not formed enough by the orthodontic tooth movement only. The purpose of this study was to investigate a new bone formation without any donor.A 3mm diameter hole was made on the femur of the mice. Then Platelet-Rich Plasma transplantation method was applied to them. The tereplag^<TM>, which was a kind of atelocollagen, was placed to this hole after soaking isolated Platelet-Rich Plasma. The new bone formation was observed in it. Then cultured periostium was placed on another hole without any transplantation, the new bone formation was not enough yet.To confirm the clinical effectiveness of the titanium mesh and the atelocollagen, The atelocollagen (Teleplag^<TM>) for the space making and the titanium mesh (M-TAM^<TM>) for the space retention were placed over the alveolar bone after making some holes on the bone. At the time of the procedure of the implantation after 6 month later, the atelocollagen was completely resorped and the alveolar bone, which was newly formed, was observed.