Significance of lymphatic invasion on regional lymph node metastasis in cancer of urinary organ by LYVE-limmunohistochemistry

2006年，我们首次用LYVE-1、D2-40、von Willebrand因子和CD31抗体等免疫组织化学方法阐明了淋巴管在肾脏中的客观分布。1.正常肾脏叶间动脉和弓状动脉周围的间质组织中淋巴管丰富。它们很少出现在肾皮质的肾小球和尿小管区。此外，在肾髓质的间质中几乎没有它们。2.在肾癌中，肿瘤周边有丰富的淋巴管，但在肿瘤内未见淋巴管。相反，肿瘤内也可见毛细血管。3.在其他病理条件下均可见淋巴管的存在，但在终末期肾癌中淋巴管密度尤为明显。2007年，我们进一步探讨了肾癌细胞淋巴管侵袭在区域淋巴结转移中的意义。肿瘤周围淋巴管密度增加，但在淋巴结转移组与无淋巴结转移组之间无明显差异。比较肿瘤大小、组织病理学分型、核异型性分级、静脉侵犯和淋巴侵犯，发现淋巴结转移组与无淋巴结转移组比较有一定差异。然而，当我们用多因素分析进一步分析这种关系时，只有肿瘤周围淋巴侵犯的因素被认为是导致淋巴结转移的因素。在进行上述研究的同时，我们申请了Lyve-1抗体的提高方法的专利。2007年初，它得到了日本政府的批准。

In 2006, we have first elucidated the objective distribution of the lymphatics in the kidney employing immunohistochemistry by LYVE-1, D2-40, von Willebrand Factor and CD 31 antibodies, which has long been obscure. 1. In the normal kidney, the lymphatics are abundant in the interstitial tissues around the interlobar and arcuate arteries. They are seldom present in the glomerular and urinary tubular regions in the renal cortex. In addition, they are almost absent in the interstitium in the renal meddula. 2. In renal carcinoma, the lymphatics are abundantly found at the periphery of the tumor, however, they are not demonstrated within the tumor itself. On the contrary, blood capillary are shown also within the tumor. 3. The lymphatics are demonstrable in the other pathologic conditions, however, among these, its density was extraordinarily noted in the end-stage kidney.In 2007, we further investigated the significance of lymphatic invasion by renal carcinoma cell on regional lymph node metastasis. The lymphatic vessel density increased around the tumor, however, it did not differ between lymph node metastasis group and negative lymph node metastasis group. When we compared the tumor size, histopathologic typing, nuclear atypism grade, venous invasion and lymphatic invasion, some differences were observed between lymph node metastasis group and node negative group. However, when we further analyzed this relationship by multivariate analysis, only the factor of lymphatic invasion around the tumor was considered to be responsible for lymph node metastasis.While we carried out the above study, we applied the patent for the raising methods of LYVE-1 antibody. In the beginning of the year 2007, it was approved by the Japanese government.