Immunohistochemical syudy on differential diagnosis of traumatic or intrinsic cerebral hematoma

1.heine oxigenase- 1 （HO-1）免疫染色在脑损伤病例中标记神经细胞和星形胶质细胞，而在对照组中不标记这些细胞。因此，HO-1免疫染色对颅脑损伤的诊断有一定的价值。血管紧张素转换酶（ACE）的免疫组化研究：ACE的免疫染色已被报道用于标记动脉硬化的不稳定斑块，在缺血性心脏病病例中使用冠状动脉切片进行了测试。在所有急性心肌梗死病例中，动脉硬化斑块中的ACE标记细胞染色。因此，我们认为ACE免疫染色可用于内源性脑出血患者脑动脉不稳定斑块的检测。外伤性脑血肿5例，内源性脑血肿5例。维多利亚蓝染色发现2例内源性脑动脉坏死，创伤性脑动脉未见坏死。淀粉样蛋白前体蛋白（APP）和神经元特异性烯醇化酶（NSE）免疫染色标记3例外伤性轴突和轴突球静脉曲张。然而，它也标记了1例脑疝的轴突球。2例内源性动脉硬化斑块中ACE标记细胞的免疫染色。然而，它也标记了1例老年妇女脑血肿的细胞。免疫染色的兴奋性氨基酸转运蛋白2（EAAT2）和胶质纤维酸性蛋白（GFAP）标记的各种星形胶质细胞，虽然没有观察到特异性的变化内在或创伤病例。提示颅内血肿的鉴别诊断应同时检查脑动脉的病理改变和脑外伤引起的轴突改变。

1.Iminunohistochemical studies on heine oxigenase-l(HO-1)Immunostaining for H0-1, one of the heat shock protein family, labelled neurocytes and astrocytes in cases of head injury, whereas it did not label those cells in control cases.Therefore, it is suggested that imniunostaining for HO-1 should be useful for diagnosing head injury.2.Immunohistochemical studies on angiotensin converting enzyme(ACE) : Immunostaining for ACE, which has been reported to label unstable plaques of arteriosclerosis, was tested using sections from coronary arteries in cases of ischemic heart diseases.In all cases of acute myocardial infarction, staining for ACE labelled cells in plaques of arteriosclerosis.Therefore, it is suggested that immunostaining for ACE should be useful for detection of unstable plaques in cerebral arteries in cases of intrinsic cerebral hemorrhage.3.Differential diagnosis of intrinsic or traumatic cerebral heniatoma Sections from cerebrum in 5 traumatic and 5 intrinsic cases of cerebral hematoma.Necrosis of cerebral arteries were detected in 2 intrinsic cases by Victria Blue staining, whereas did not in traumatic cases.Immunostaining for amyloid precursor protein(APP)and neuron-specific enolase(NSE)labelled varicose axons and axonal bulb in 3 traumatic cases.However, it also labelled axonal bulb in 1 intrinsic case with cerebral herniation.Immunostaining for ACE labelled cells in plaques of arteriosclerosis in 2 intrinsic cases.However, it also labelled cells in 1 intrinsic case with cerebral hematoma of an old woman.Immunostaining for exitatory amino acid transporter 2(EAAT2)and glial fibrillary acidic protein(GFAP)labelled various astorcytes, although no specific changes to intrinsic or traumatic cases were observed.It is suggested that both examinations of pathological changes of cerebral arteries and examinations of axonal changes due to head injury should be necessary for differential dignosis of intrinsic or traumatic cerebral hematoma.