High resolution mass spectrometer coupled with nanoLC and ion mobility spectrometry

我们申请了一个高分辨率的纳米液相色谱-质谱联用离子迁移谱仪和并行积累和串行碎裂技术。它将主要用于从高度复杂的混合物中测定代谢物和肽。特别是，该仪器将为现有的面板增加更多的分析工具，从而能够以更高的灵敏度和选择性识别特别低浓度的分析物，同时缩短测量时间。因此，所提出的仪器的主要应用领域是（高通量）代谢组学和蛋白质组学领域。该仪器将被纳入该研究所的质谱中心，该中心的几台液相色谱耦合、低分辨率和高分辨率质谱仪已经在全天候运行。然而，这些工作组已经完全占据了。申请的设备将进一步缓解现有设备的压力，从而满足对测量时间和额外分析工具的不断增加和不断增长的需求，特别是在合作项目的背景下。具备操作和维护要求（人员、房间）。还提供了用户指南。有了所要求的设备，洪堡医学院质谱中心在低分辨率和高分辨率质谱以及液相色谱方面已经积累的专业知识将进一步增长，这将有利于当前的研究合作，特别是未来的合作。

We apply for a high-resolution nano liquid chromatogrphy coupled mass spectrometer with an ion mobility spectrometer and parallel accumulation and serial fragmentation techniques. It will be used mainly for the determination of metabolites and peptides out of highly complex mixtures. In particular, the instrument will add further analytical tools to the existing panel, thereby enabling the identification of especially low-concentration analytes with increased sensitivity, selectivity and, at the same time, shorter measurement times. Accordingly, the main areas of application for the proposed instrument are the fields of (high throughput) metabolomics and proteomics. The instrument will be integrated into the Mass Spectrometry Center of the Institute, where several liquid chromatography-coupled, low-resolution and high-resolution mass spectrometers are already in operation around the clock. However, these are already completely occupied by the working groups. The device applied for would additionally relieve the existing devices, so that the increased and constantly rising demand for measurement times and additional analytical tools can be met, especially in the context of cooperation projects. Requirements (personnel, rooms) for operation and maintenance are available. A user guideline is also available. With the requested device, the expertise in low- and high-resolution mass spectrometry as well as liquid chromatography already built up at the Mass Spectrometry Center of the Medical Faculty at the Homburg site will further grow, which will be beneficial for current research collaborations and especially future ones.