Suppression of exercise-induced anaphylaxis by intake allergen with some food components

卵清蛋白（OVA）致敏BALB/c小鼠或溶菌酶（Ly）致敏B10。用小鼠研究食物依赖性运动致过敏反应的机制。口服注射OVA致敏小鼠的过敏反应症状和急性运动责任明显高于未给予OVA的跑步机运动小鼠。口服Ly致敏小鼠和急性运动责任小鼠相比，Ly致敏小鼠的过敏原通过胃肠道损伤渗漏并到达肝脏。需要过敏反应来评估全身症状，并通过观察一些典型症状如抓挠、摩擦和发绀来评分。然而，评分依赖于主观观察，导致很少对过敏反应进行定量评价。观察致敏小鼠15小时的运动和自主体力活动。口服Ly后致敏小鼠的自主体力活动明显减少，急性运动责任明显减少。在致敏小鼠模型中发现了过敏反应症状评分与小鼠直肠温度或自愿体力活动之间的相关性。对致敏小鼠长期注射果胶或乳酸饮料可抑制小鼠自主体力活动的减少。这些结果提示果胶或乳酸饮料对致敏小鼠的过敏症状有保护作用。

Ovalbumin(OVA) sensitized BALB/c mice or lysozymes(Ly) sensitized B10.A mice were used to investigate the mechanisms of food dependent exercise-induced anaphylaxis. Anaphylaxis symptoms of OVA sensitized mice were clearly observed after OVA injection orally and acute exercise responsibility than that of mice treadmill exercised without OVA administration. Allergen was leaked through gastrointestinal damages and reached to a liver of Ly-sensitized mice after Ly injection orally and acute exercise responsibility than unsensitized mice. Anaphylaxis is required to evaluate the systemic symptoms, and is scored by observation of some typical symptoms such as scratching, rubbing and cyanosis. However, the scoring depends on subjective observation, resulting in the poorly quantitative evaluation for anaphylaxis rarely. Locomotion and voluntary physical activity of sensitized mice were examined for 15hr. Voluntary physical activity of sensitized mice was significantly decreased after Ly injection orally and acute exercise responsibility as same as locomotion decrease. The correlation between the symptoms scoring of anaphylaxis and rectal temperature or voluntary physical activities of the mouse was recognized in sensitized mouse model.Long time injection of pectin or a lactic acid drink to sensitized mice was suppressed their voluntary physical activity decrease. These results suggested that pectin or a lactic acid drink have protective effect for sensitized mice symptoms of anaphylaxis.