Production of wine with high resveratrol content using non-vinifera grapes

已知白藜芦醇在葡萄皮和种子中积累，以应对环境压力，如真菌感染。白藜芦醇是一种多酚，具有显著的生物功能，包括抗癌活性。在本研究中，我们的目标是通过将葡萄暴露在一种环境胁迫UV-C中，诱导白藜芦醇的产生，从而生产出高含量的白藜芦醇葡萄酒。紫外- c辐射对葡萄产生白藜芦醇的诱导作用用波长为253.7nm的紫外- c辐射葡萄，照射距离为10cm。辐照10 ~ 20min诱导效果最好，可使葡萄皮中白藜芦醇含量提高到0.5mg/g鲜重。过滤膜对葡萄酒中白藜芦醇含量测定的影响，研究了过滤膜对白藜芦醇的吸收。醋酸纤维素膜对白藜芦醇具有较强的吸附能力，而PTFE膜和氧化铝膜对白藜芦醇的吸附能力较弱。还原剂对白藜芦醇含量的影响白藜芦醇在葡萄汁和葡萄酒中容易被氧化。在所使用的还原剂中，亚硫酸盐（二亚硫酸钾）对白藜芦醇的氧化作用最好。光对酒瓶中白藜芦醇异构化的影响众所周知，白藜芦醇是在葡萄中以捕集剂形式合成的。然而，在葡萄酒中检测到相当数量的顺式白藜芦醇。为了确定白藜芦醇在葡萄酒生产过程中异构化的原因，研究了光照对异构化的影响。白藜芦醇在荧光照射下容易异构化。

Resveratrol is known to accumulate in grape skin and seed in response to environmental stress such as fungal infection. Resveratrol is a polyphenol having noteworthy bio-functional activities, including anti-cancer activity. In this research, we aim to produce wines with high resveratrol content by exposing grapes to UV-C, a kind of environmental stress, to induce reveratrol production.1. Induction of resveratrol production in grapes by UV-C irradiationGrapes were irradiated with UV-C (wavelength : 253.7nm) at a distance of 10cm. Maximum induction was achieved by irradiation for 10-20min, and resveratrol content in grape skin was increased to 0.5mg/g fresh weight by this treatment.2. Effect of filter membrane on measurement of resveratrol in wineThe absorption of resveratrol to filter membrane was investigated. Cellulose acetate membrane strongly absorbed resveratrol, whereas PTFE and aluminum oxide membrane did not.3. Effect of reducing agent on resveratrol contentResveratrol was easily oxidized in grape juice and wine. Among the reducing agents used, sulfite (potassium disulfite) best prevented the oxidation ofresveratrol.4. Effect of light on isomerization of resveratrol in wine bottleIt is known that resveratrol is synthesized in the traps form in grape. However, a considerable amount of cis-resveratrol was detected in grape wine. To determine the cause of isomerization of resveratrol during wine production, the effect of light was investigated. Resveratrol in a white-clear bottle was isomerized easily upon fluorescent light irradiation.