High resolution LC-MS/MS device

该应用程序用于高分辨率质谱仪，包括上游HPLC系统和控制和数据评估所需的软件。该设备旨在识别和量化药物，包括用于治疗的蛋白质。此外，蛋白质组分析计划阐明病理机制或寻找生物标志物。在临床研究背景下的药代动力学参数的测定，目前的设备主要局限于小分子，将扩大到包括治疗性蛋白质，特别是抗体。在过去的几年里，抗体变得越来越重要，而且不仅限于肿瘤治疗和自身免疫性疾病的治疗。定性研究也特别有趣。因此，研究蛋白质的糖基化模式对其药效学和动力学的影响具有特殊的意义。临床药理学研究所在检测和确定废水中的药物和非法药物方面具有长期的专门知识，以便找到与环境毒理学和公共卫生问题有关的答案。在这个研究领域，专业知识将扩大到包括非目标分析，即鉴定最初在样品中没有怀疑的物质。此外，污水净化新工艺产生的转化产物的鉴定和污水倾倒的作物植物中药物代谢物的鉴定是一个进一步的研究课题。为此，数据独立的测量是必要的（DIA，数据独立采集；在不预先选择某些质量的情况下，对测量范围内的所有离子进行碎片化和分析）。德累斯顿大学医院部门的几个工作组，以及药理学和毒理学研究所的工作组，对使用所要求的设备进行蛋白质组分析很感兴趣。来自细胞培养和动物模型的样本以及临床样本将在各种项目中进行分析。目标是更好地理解细胞发育过程和通信通道，以及寻找新的生物标志物和可能的治疗有用的靶点。蛋白质组分析需要确定精确的质量和DIA模式的可用性。

The application is for a high-resolution mass spectrometer including an upstream HPLC system and the software required for control and data evaluation. The device is intended to identify and quantify drugs, including therapeutically used proteins. In addition, proteome analyses are planned to elucidate pathomechanisms or to search for biomarkers.The determination of pharmacokinetic parameters in the context of clinical studies, which is largely limited to small molecules with the current device, is to be expanded to include therapeutic proteins, in particular antibodies. In the past years, antibodies become more important and are not limited to oncology therapy and the treatment of autoimmune diseases. Qualitative studies are also of special interest. Therefore, investigations with regard to the influence of the glycosylation pattern of proteins on their pharmacodynamics and kinetics are of special interest.At the Institute of Clinical Pharmacology, there is a long lasting expertise in detection and determination of drugs and illegal drugs in wastewater in order to find answers related to environmental toxicological and public health issues. In this research area, the expertise is to be expanded to include non-target analysis, i.e. the identification of substances initially not suspected in the sample. In addition, the identification of conversion products caused by new wastewater cleaning procedures and of drug metabolites in crops plant poured with wastewater are a further research topic. For this purpose, data-independent measurements are necessary (DIA, data independent acquisition; fragment and analyse all ions in a measurement range without pre-selecting certain masses).Several working groups at departments of the University Hospital Dresden, as well as working groups at the Institute of Pharmacology and Toxicology, are interested in using the requested device for proteome analyses. Samples from cell culture and animal models as well as clinical samples are to be analysed in various projects. The goals are a better understanding of cellular development processes and communication channels as well as the search for new biomarkers and possible therapeutically useful targets. Proteome analysis requires the determination of exact masses and the availability of the DIA mode.