Dynamics and enviromental response of insect hormones controling metamorphosis and diapause

岩上等人。发现家蚕毒素基因由30个拷贝的相关基因组成，并确定了家蚕毒素产生细胞所特有的顺式元件。Ichikawa等人。成功监测神经分泌细胞电兴奋2~5d。产生相同神经肽的这些细胞的电兴奋由其他中枢神经元同步和调节。Yamashita et al.利用转基因果蝇鉴定了滞育激素基因的顺式元件，该元件由650bp组成。Shimada等人。确定了PERA、ECR和E75基因的基因座。Aizono等人。发现5-羟色胺调节促胸腺激素(PTTH)的释放对甘薯夜蛾从蛹滞育中唤醒非常重要。Mizoguchi等人。建立了家蚕血淋巴中微量甲状旁腺素的定量方法，并测定了家蚕血淋巴中甲状旁腺素滴度的发育变化。Kataoka等人。用表达克隆的方法鉴定了甲状旁腺素受体的候选基因。该受体属于G蛋白偶联受体家族。Fujiwara等人。发现早晚基因BHR和晚基因urbain在家蚕无翅突变体中的表达水平很低。Yaginuma等人。鉴定了ECR的7种异构体。

Iwami et al. found that bombyxin gene is composed of 30 copies of related genes and identified a specific cis element for bombyxin-producing cells. Ichikawa et al. succeeded in monitoring electrical excitation of neurosecretory cells for 2-5 days. The electrical excitation of these cells producing the same neuropeptide was synchronized and regulated by other central neurons. Yamashita et al. identified a cis element composed of 650 bp for diapause hormone gene by using transgenic fruit fly, Drosophila. Shimada et al. identified gene loci of period, EcR and E75 genes. Aizono et al. found that prothoraicotropic hormone (PTTH) release regulated by serotonin is very important for arousal of sweet potato hornworm from pupal daiapause. Mizoguchi et al. developed a method to quantify a minute amount of PTTH and measured developmental changes of PTTH titer in hemolymph of Bombyx mori. Kataoka et al. identified a candidate of PTTH receptor cDNA by using expression cloning method. The receptor is belong to a family of G-protein coupled receptor. Fujiwara et al. found that expression levels of early late gene, BHR and late gene, urbain were very low in the wingless mutant of Bombyx mori (fl). Yaginuma et al. identified 7 isoforms of EcR.